| Background: Keloids are benign tumours of the skin. They tend to occur in younger patients after different kinds of injuries, infection or they may develop spontaneously. In contrast to hypertrophic scars, keloids are not confined to the original wound, but grow into the corresponding healthy skin .The rarely recede within time. Most patients complain of itching and suffer from impairment of their quality of life. Only little is known about the pathogenesis of keloids, although they have been clinically well characterized for a long time. Successful treatment of keloids is difficult because of frequent recurrences. Fibroblasts are isolated form keloids tissue and maintained in cell culture continue to express an increased capacity to proliferate and to produce collagen. Nowadays, one of the focus problems of keloids is how to inhibit these actions of keloid fibroblast,then find effective ways to treat keloids. Chitosan and its derivatives exhibit myriad biological actions, such as antitimicrobial, biodegradability, biocompatibility and accelerated wound healing properties. So it becomes an optimal biological dressing of articial skin. But there are few reports on their fuction on keloid and KFB.Objective: To investigate the effects of CM-CH on keloid fibroblasts proliferation and collagen synthesis.Methods: Keloid fibroblasts were isolated from fresh keloid tissue and cultured. The KFB(from the 3rd to 9th passages)were treated with various concentrations of CM-CH. Triamcinolone acetonide was used as appositive control, cell culture solution was used as a negative control. The effect of CM-CH on proliferation was examined by MTT and cell count technique. Human type III pre-collagen was determined with radio-immunoassa measurement and hydroxy proline (HP), was evaluated by colorimetric analysis. After CM-CH affected the KFB. The infuence of KFB on CM-CH film was observed.Results: The MTT results showed that CM-CH at lower concentration (10μg/ml, 50μg/ml, 10μg/ml) after treating 24h, did not inhibited KFB proliferation significantly (p>0.05). After treating 48h and 72h, all of the three concentrations showed inhibition effects significantly (p<0.05). At higher concentration (200μg/ml) the significant inhibition effect on KFB proliferation was observed at any detected time (p<0.05). Thisinhibition of CM-CH on KFB was not only the concentration but experiment time as well. The inhibition effect of CM-Ch on KFB is similar to that of triamcinolone acetonid (p>0.05) at 200μg/ml concentrations after 24h, 48h, 72h. The result of cell count technique is similar to MTT. The result of colorimetric analysis of hydroxy proline (HP) indicated that CM-CH experiment concentrations after 48h on KFB could markedly decrease the synthesis of collagen (p<0.01). But the inhibition effect of the synthesis of collagen was significantly different than that of triamcinolone acetonid (p<0.01). The result of human typelll pre-collagen determined with radio-immunoassa measurement showed that CM-CH at lower concentration (10μg/ml, 50μg/ml) did not decrease the content of III pre-collagen (p>0.05). The content of III pre-collagen was at lower level when CM-CH at high concentrations. (100μg/ml, 200μg/ml)(p<0.05) and showed no significant difference than that of triamcinolone acetonid.Conclusion: CM-CH can inhibit the proliferation of KFB. The inhibition of CM-CH on KFB was not only the concentration but experiment time as well. CM-CH can inhibit the synthesis of collagen of KFB.in vitro by a concentration-depended manner, indicating that CM-CH may be effective in treatment of keloids.Postgraduate: Chen Hairong (dermatology ) Directed by: Professor Wu Yanfang...
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