Study On The Relation Between Composition And Pharmacology Of Ge Gen Qin Lian Decoction

Objective:To explore the composition principles of GGQLD through experiment of bacteriostasis in vitro -. antipyretic % anti-diarrhea and bacteriostasis in vivo of animal model of shigella.MethodscTo observe different pharmcological effects of sixteen compounds after they were given by measuring mininal inhibitory concentration(MIC) through double-fold dilutions method and diameters of inhibitory circles through the method of drilling hole on agar plate against staphylococcus aureus, Escherichia coli. shigella flexnery 2 a and streptococcus pneumoniae,average most temperature response highness( A T) and temperature response index of six hours(TRl6) after the accomplishment of rat model of Pyrexia by Suspension of Escherichia coli,diarrhea index(DI^ fecal level after accomplishment of diarrhea animal model,small intestine drive rate and bacteriocolony number of colon content and tissue and pathomorphology change after the accomplishment of rabbit shigella model. Results: (DCCF has the most bacteriostasis in vitro while different bacteriostasis effects as to different strains,and next SBG,GWF has a little bacteriostasis against shigella flexnery 2 a and staphylococcus aureus. and PLO against staphylococcus aureus.When combinating with CCF,the other three drugs have counteracter against CCF.As to Escherichia coli,GWF has more counteracter effect against CCF than PLO.GWF can counteracter against SBG while PLO not.As to streptococcus pneumoniae, GWF has more counteracter effect against CCF than PLO.GWF and PLO has no counteracter against SBG.As to shigella flexnery 2 a ,SBG has more counteracter against CCF than PLO and GWF.GWF can counteract against SBG while PLO not,GWF and PLO can enforce each other.As to staphylococcus aureus,GWF and PLO have no counteracter against SBG,but they can enforce each other.In the method of drilling hole on agar plate,PLO can enforce SBG.(2)`BG has the most antipyretic effect, As to TRI6,CCF and GWF have counteracter against SBG,but PLO can enforce.PLO has no effect on CCF and GWF,but GWF can counteract against CCF.As to average most temperature response highness,CCF and GWF have counteracter against SBG,but PLO can enforce.GWF has no effect on CCF and PLO,but PLO can counteract against CCF.(3)As to DI,CCF can reduce DI,.SBG can counteract against CCF but PLO and GWF can enforce.PLO can enforce SBG,but GWF can counteract it.GWF has no effect on PLO.As to fecal level,CCF can reduce fecal level,.SBG can counteract against CCF but GWF can enforce while PLO has no effect on it.PLO can enforce SBG,but GWF can counteract itGWF has no effect on PLO.(4)CCF and GWF can reduce drive rate.SBG and PLO has counteracter against CCF but not against GWF, while GWF can enforce CCF.(5)In shigellosis rabbit model,CCF and SBG have bactericidal effect,while GWF and PLO not.but SBG has counteracter against CCF.when thinking of colonic pathologic change,GWF has significant effect on reducing inflammation,and other drugs has no significant effect on it,also they have no significant intersect on each other.Conclusions:Some of GGQLD's pharmcological effect as antipyretic, anti-diarrhea and bactericidal in vivo and in vitro,the main drug that corresponding to different pharmcological effects is different,and other drugs have counteracter or enforcement effect while combinating with them.