Detection Of Neisser Ia Gonorrhoeae By The Culture And The Real-time Fluorescence Quantitative Polymerase Chain Reaction

Objective To detect the DNA of Neisseria gonorrhoeae in clinical genitourinary specimens by the real-time fluorescence quantitative Polymerase Chain Reaction (FQ-PCR) , and to provide a reliable support for the clinical diagnosis and treatment of gonorrhoea.Methods In 173 clinical genitourinary specimens .Neisseria gonorrhoeaes' DNA were detected by Neisseria gonorrhoeae real-time fluorescence PCR diagnostic kit with PE5700 automatic fluorescence PCR analysis instrument. At the same time , the specimens were isolated by T-M medium with PolyVitex , identified by ATB Expression and the matched API NH . Agent susceptibility test was determined by ATB Expression and the matched ATB NH. The P -lactamase of the Neisseria gonorrhoeaes were detected by acidity paper method ,Results Of 173 clinical specimens , with real-time fluorescence quantitative PCR , 42 cases were positive and the positive ratio was 24. 3%, among them, 23 cases were male urethra secretions, 18 were female womb secretions , 1 was prostate fluid; with the germiculture method ,41 cases were positive and the positive ratio was 23. 7%,among them ,22 cases were male urethra secretions, 18 were female womb secretions, was prostate fluid.When compared to the germiculture, the specifity of FQ-PCR was 100%, sensitivity was 97. 6%, the agreement was 99.4%.Conclusion In detecting Neisseria gonorrhoeaes , the sensitivity of FQ-PCR is higher than that of germiculture , FQ-PCR is a simple , rapid , and specific method to analyze Neisseria gonorrhoeaes, it is of potential use for clinical diagnosis. But at the same time of germiculture, the agents susceptibility test andP -lactamases can be detected .