| Objective:1.Collected the clinical data of patients with gastric cancer within2009-2011which havebeen tested the expression status of Her-2in gastric carcinoma by immunohi stochemi stry (IHC),analysis the relationship of Her-2expression status of gastric cancer patients with patient age,gender, and cancer tissue site, degree of differentiation, the general type, depth of invasion andlymph node metastasis, explore significance of Her-2expression status in the treatment andprognosis of patients with gastric cancer.2.Collected the gastric cancer histopathology wax block of gastric carcinoma patientswithin2009-2011which heve been detected expression status of gastric cancer tissues byimmunohi stochemi stry (IHC),detect the Her-2expression of gastric cancer tissues by real-timefluorescence quantitive polymerase chain reaction (RTQ-PCR) method,explore new,shortcutsimple, objective, eiffcient, cost-effective detection method for the detection of gastric cancerpatients with Her-2gene expression.Method:1.Collected the clinical data of71patients with gastric cancer within2009-2011whichhave been tested the Her-2expression status in gastric carcinoma by immunohi stochemi stry(IHC), to understand its expression rate, group by the patient’s age, sex, site of the cancerorganizations,degree of differentiation, the general type, depth of invasion, clinical indicatorsand with or without lymph node metastasis, and analyze the statistical significance by SPSS17.0statistical software.2.Detect Her-2expression in gastric cancer tissues paraffins of71cases gastric cancerpatients using real-time fluorescence quantitive polymerase chain reaction (RTQ-PCR),applingSPSS17.0statistical software do statistical analysis of immunohistochemistry and real-time PCRwith matching design method.Results:1. Her-2expression detected by immunohistochemistry in gastric cancerHer-2overexpression rate was22.5%in71cases of gastric carcinoma， of which Her-2overexpression rate was20.9%(9/43) in=60-year-olds，〉60-year-olds was25%(7/28)； male group was23.6%(13/55)， the female group was18.8%(3/16)；cardiawas35.7%(10/28), gastric antrum was26.7%(4/15)， gastric body was7.1%(2/28)；in the poorly differentiated group was21.6%(8/37)， well differentiated was23.5%(8/34)； type of ulcer was24.3%(9/37)， fee ulcer type was20.6%(7/34)；25%(10/40)in no invaded serosa group, invaded serosa group was19.4%(6/31)； with lymph nodemetastasis was48%(12/25), without lymph node metastasis was8.7%(4/46)； earlygastric cancer group (I/II period) was10.9%(5/46)， advanced gastric cancer group(III/IV period) was55%(11/25). Her-2overexpression have significant correlation(p<0.05) with gastric cancer site, lymph node metastasis and TNM staging, haveno significant correlation (P>0.05) with age, gender, degree of differentiation,the general type and depth of invasion.2.Her-2expression detected by real-time lfuorescence quantitive polymerase chainreaction (RTQ-PCR) in gastric cancerHer-2overexpression rate was19.7%in71cases of gastric carcinoma， of whichHer-2overexpression rate was16.3%(7/43) in=60-year-olds，〉60-year-olds was25%(7/28)； male group was21.8%(12/55), the female group was12.5%(2/16)；cardiawas32. l%(9/28), gastric antrum was20%(3/15), gastric body was7.1%(2/28)； inthe poorly differentiated group was21.6%(8/37), well differentiated was17.6%(6/34)； type of ulcer was21.6%(8/37), no ulcer type was17.6%(6/34)；22.5%(9/40) in no invaded serosa group, invaded serosa group was16.1%(5/31)； withlymph node metastasis was40%(10/25), without lymph node metastasis was8.7%(4/46)；early gastric cancer group (I/II period) was8.7%(4/46), advanced gastric cancergroup (III/IV period) was40%(10/25).3.Comparison of real-time lfuorescence quantitative PCR and immunohistochemicaldetection of Her-2expressionHer-2expression status in71cases of paired design organization paraffin, usingimmunohi stochemi stry Her-2over expression is16(22.5%) cases,using real-time fluorescencePCR Her-2overexpression is14(19.7%) cases, positive consistent rate is76.5%(13/17),negative concordance rate was93.1%(54/58), total concordance rate was94.4%(67/71),testionresults of the two methods have no significant difference (P>0.05).Conclusions:1-. Her2expression increased in gastric cancer be closely related to gastric cancer location,lymph node metastasis and TNM stage, to understand Her-2expression status in gastric cancer tissues have important clinical significance for treatment efifcacy,evaluation and prognosisjudgment of patients with gastric cancer.2. The detection results of real-time fluorescence quantitive PCR andimmunohi stochemi stry is no significant difference, and real-time fluorescence quantitive PCR inregard to the immunohi stochemi stry and fluorescence in situ hybridization is simple, objective,efficient and cost performance, be expected to become an new option in addition toimmunohi stochemi stry and fluorescence in situ hybirdization in the Her-2detection of gastriccancer parafifn-embedded tissues. |
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