| Hyperlipidemia and thrombosis involved in the process of atherosclerosis(AS). Epidemiologic studies showed that hyperlipidemia was closely correlation with hypercoagulability. Our laboratory previous studies have certified that very low density lipoprotein (VLDL), low density lipoprotein (LDL), high density lipoprotein (HDL) were oxidized in endogenous hypertriglyceridemia(HTG) patients. To explore the relationship between plasma lipoprotein and hypercoagulable state, based on our laboratory and abroad studies, we further researched the influence of Ox-VLDL, Ox-LDL and Ox-HDL on blood coagulation , fibrinolysis and Platelet Aggregation.Plasma VLDL, LDL and HDL from 21 endogenous hypertriglyceridemic patients and 21 normal subjects were isolated by the density gradient ultra-centrifugation method. Plasma triglycerides (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDLC) were measured by enzyme method. The oxidation of LDL, VLDL and HDL was identified by agarose gel relative electrophoretic mobility (REM), absorbance at 234nm (A234nm, conjugated diene, CD) and thiobarbituric acid reaction substances (TBARS) contents. The results showed that the plasma TG, TBARS contents level in HTG groups were 1.6 and 0.4 times of the control groups respectively (P<0.01). The plasma HDLC in HTG groups was 32% lower than that of the control groups (P><0.01). The relative electrophoretic mobility (REM), absorbance at 234nm and TBARS contents of VLDL, LDL and HDL in HTGgroups were significantly higher than those of the control groups (P<0.01). These results suggest that VLDL, LDL and HDL were oxidatively modified in endogenous hypertriglyceridemia patients in vivo.In order to know the influence of VLDL, LDL and HDL in endogenous hypertriglyceridemia patients on blood coagulation, fibrinolysis and Platelet Aggregation, with the reaction system which consisted of mixed fresh normal plasma, prothrombin time (PT), activated partial thrombplastin time (APTT), plasminogen activator inhibitor 1 (PAI-1) and tissue plasminogen activator (t-PA) were measured according to the direction of the kits. Platelet Aggregation which was induced by ADP was performed and recorded on a 4-channel light Aggregometer. The results showed that the PT and APTT of VLDL , LDL and HDL in HTG groups were obviously shorter than that of the control groups (P<0.05). The VLDL in HTG groups was higher in activity of t-PA and lower in activity of PAI-1 than that of the control groups (P<0.01), but activity of t-PA and activity of PAI-1 in LDL and HDL were no difference between in HTG groups and in the control groups. The Platelet Aggregation of VLDL, LDL and HDL in HTG groups were significantly stronger than that of the control groups (P<0.01). The correlation analysis indicated that relative electrophoretic mobility of VLDL and HDL in HTG groups was negatively correlated with PT (r= -0. 8012 and -0. 5641, P<0.01). These results suggest that HTG-VLDL, HTG-LDL and HTG-HDL enhance the activity of blood clotting system and Platelet Aggregability, but only HTG-VLDL affect the activity of fibrinolysis system.In order to probe whether the effects of plasma lipoprotein on bloodcoagulation, fibrinolysis and Platelet Aggregation in endogenous hypertriglyceridemia patients were correlation with oxidative modification of lipoprotein. Plasma VLDL, LDL and HDL in normolipemic donor were isolated by the density gradient ultracentrifugation method. VLDL, LDL and HDL were oxidatively modified by Cu2+ and HOC1. Ox-VLDL, Ox-LDL and Ox-HDL were added to the reaction system which consisted of mixed fresh normal plasma, prothrombin time (PT), activated partial thrombplastin time (APTT), plasminogen activator inhibitor 1 (PAI-1) and tissue plasminogen activator (t-PA) were measured according to the direction of the kits, Platelet Aggregation which was induced by ADP was performed and recorded on a 4-channel light Aggregometer. The results showed that the relative electrophoretic mobility (REM), absorban... |
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