| Objictive: to investigate the roles of lymphocytes in regulation of endometrial stroma cells decidualization and embryo implantation.Methods:1.Stroma cells derived from human endometrium during the late proliferate phase or secretive phase were treated with estradioK progesterone to induce decidualization. Peripheral blood lymphocytes(PBL) isolated from women in the luteal phase and early pregnancy were added to to the stroma cells. The stroma cells morphologic changes and prolactin (PRL)production in the media were examined and compared.2. The 4-day-mouse embryo were added to the stroma cells after cultured 8 days. The hatching, adhereing, outspreading of mouse embryo and the development of stroma cells were observed under the microscope.Results: 1. PBL derived from early pregnancy women significantly increased the degree of the stroma cells decidualization compared with PBL derived from luteal phase and the control group.2. PRL secretion of co-cultured with PBL isolated from early pregnancy women was significantly higher than the control group(p<0.05). Wheras the significantly augmentation of PRL prouduction was not observed in co-cultured with PBL isolated from women in luteal phase. 3.PBL isolated from luteal phase women and early pregnancy women significantly promoted the embryo hatching, adhering, outspreading on the endometrial stroma cells(p<0.05),esperialy the hatching and adhering(p<0.01). PBL isolated from early pregnancy women significantly promoted embryo outspreading compared with PBL isolated from luteal phase women(p<0.05).conclusion:1.The lymphocytes derived from early pregnancy can contribute to the stroma cells decidualization and particularly improve PRL secretion.2. Lymphocyte isloted from early pregnancy can promote the embryo outspreading in co-cultured with endometrial stroma cells. .3. The lymphocytes derived from early pregnancy may promote the embryo implantation by improving PRL secretion based on stroma cellsdecidualization regulated by E, P.
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