Identification Of The Genuine Drug Polygonatum Odoratum In Hunan By RAPD

Yuzhu is the rhizome of Polygonatum odoratum (Mill.) Druce (Fam. Liliaceae),which is not only a common Chinese drug but also a genuine drug in Hunan. Many research methods ,such as morphological identification,anatomical identification and physical and chemical identification,have been applied in the study on Yuzhu. The molecular evidence has not been reported in identification of Yuzhu yet. The technique of random amplified polymorphic DNA(RAPD) ,as a DNA molecular index , was used in the study which included not only Yuzhu from different habitats in Hunan,Guandong and Beijing but also Polygonatum cyrtonema Hua and Polygonatum stenophyllum . In this study, the establishment of the RAPD fingerprinting of Yuzhu was performed,which included the optimised ingredients of the RAPD and selected the random primers. The data based on the RAPD were used to generate Nei similarity coefficients and to construct a tree diagram using the UPGMA in the NTSYS programs .The results and conclusions : (1) The RAPD reaction system suitable for Yuzhu and Huangjing was established; (2) 17 primers were screened from 46 random 10-mer primers, and a totle of 136 DNA bands were amplified ,131 of which (96.3%) were polymorphics; (3) It was possible that of all the samples could be identified by only one primer ; (4) The RAPD results reach the same conclusions as those obtained from the studies on morphology and cytology; (5) There was a rich genetic polymorphism between Yuzhu and Huangjing. It was evident to see the genetic difference between the genuineness of Yuzhu and the different habitats of Yuzhu. It was the first time to apply the RAPD technology to identify Yuzhu and Huangjing, which was a beneficial try to add a new method into the Chinese traditional medicine identification...