The Expression And Function Of IRP2mRNA,FnmRNA,TfRmRNA In Rat Intestine With Iron Deficiency

Iron is an important element of body , the balance of iron metabolism is important to retain the normal function of cell. The maintenance of iron home ostasis is accomp- lished mainly by the cooperation and the coordination of so me protein such as Ferri - tin( Fn) -, Transferrin Receptor (TfR) and Iron Re gulatory Protein (IRP) et al.. It has been found that the expression of TfR and Fn are regulated by IRP at the level of post-transcriptional. At present, two f orms of IRP have been known, namely IRP1 and IRP2.The absorption of iron in intestine is an important of iron metabolism in the body. In recent years, the studyof the iron absorption and regulation empha sized on the IRPi, which regulates the expression of TfR and Fn at the level of post- transcriptional. However, less has been done as to IRP2. It was not yet reported about the study of the expression of IRP2mRNA -. Fn mRNA -, TfR mRNA and their correlation combining with different iron deficiency, and the mechamism of iron absorption has not been clarified completely.In our study, we established the animal model of rats with nutritional iron deficiency and detected the expression of IRPamRNA -, FnrnRNA, TfRmRNA in duodenum membrane of the model to discuss the function of IRPa at genetic level. At the same time, we also researched the correlations among IRP2-mRNA, FnmRNA and TfRmRNA , to discuss the function of the IRP2 , Fnand TfR in iron metabolism of intestine, So to explore the metabolism of intestine .Method:Select forty healthy adult Wistar rats, and randomly divided them into con ±trol group and iron deficiency group. The model of rat with nutritional iron deficiency was established by the Liao Qing Kui . From fifteen to sixty days, iron deficiency and IDA gradually occurs in the rats. We collected 1.5 millili± ter bloods from orbital vein plexus of each rat and obtained the serum samples to detect the serum iron (SI), serum ferritin (SFn). According to the concentration of SI, SFn and the rats were divided into four groups: control group, the group of recessive iron deficiency , the group of mild iron deficiency anemia, the group of moderate iron deficiency anemia. The samples of the duodenum membrane of rat were freezingly preserved at -70 ℃. By the instruction of the Test Kit, the total RNA of the sample was extracted, and then preserved in liquid nitrogen. Freezingly .The expression of IRP2 mRNA , FnmRNA and TfR± mRNA. of samples were detected by the RT±PCR . sFn was measured by Che ±miluminescence Assay and SI was measured by flame Assay ,etc. Result(1) IRPimRNA: The expression of IRPimRNA in the central group was 0.38 ±0.12, the group of recessive iron deficiency was 0.39 ± 0.16, the group of mild iron deficiency anemia was 0.51 ±0.08 , the group of moderate iron deficiency anemia was 0.51±0.14.There was no difference between recessive iron deficiency group and central group (P>0.05) ; The expression of IRP2mRNA in mild iron deficiency anemia group and moderate iron deficiency anemia group were significantly higher than that of contra! group (P<0.05) .Both recessive iron deficiency group and moderate iron deficiency anemia group were no difference with mild iron deficiency anemia group ( P>0.05 ) ; There was significant difference between recessive iron deficiency and moderate iron deficiency anemia group ( P<0.05 ) .In a word, from the potential of the all and the one, the expression of IRP2mRNA was increased as iron deficiency increased.(2) TfRmRNA: The expression of TfRmRNA was increased as iron deficiency increased. The expression of TfRmRNA in Contra! group was 0.43 ±0.14, recessive iron deficiency group was 0.55+0.10, mild iron deficiency anemia group was 0.70 ±0.08 and moderate iron deficiency anemia group was 0.78. ±0.15. There were significant difference between every two group (P<0.05) .(3) FnmRNA: The expression of FnmRNA was decreased as the increasing of iron deficiency. The expression of FnmRNA in central group was 1.02 ±0.20, recessive iron deficiency group was 0...