Expression And Significance Of NO/NOS In Endometrium Of Anovalatory Functional Bleeding

Objective: The purpose of this study was to investigate the expression of eNOS, iNOS in the endometrium of patients with anovulatory functional bleeding and their correction with serum estrogen and progestin, and to explore their roles in pathogenesis of anovulatory functional bleeding.Methods: RT-PCR was used to study the expression of eNOSmRNA, iNOSmRNA and immunohistochemistry for eNOS protein, iNOS protein in 10 normal endometrium and 36 endometrium of anovulatory functional bleeding. Serum estrogen and progestin were analyzed by automatic particle chemiluminescence immunization analysis system. Results:1. eNOS was detected in the glandular epithelial cells and the endothelialcells. INOS was detected in the glandular epithelial and some of the interstitial cells.2. The expression of eNOSmRNA, eNOS protein was higher in the endometrium of simple hyperplasia and complex hyperplasis than those in normal endometrium, so was the expression of iNOSmRNA, iNOS protein in the proliferative endometrium ofanovulatory functional bleeding.3. There was no significant difference between the patients of anovulatory functional bleeding and normal persons in terms of serum estrogen. But in the patients, the level of serum progestin was significantly lower than that of normal persons.4. There is no correlation between the expression of eNOAmRNA or iNOAmRNA and serum progestin or estrogen in both the patients of anovulatory functional bleeding and normal persons.Conclusions: eNOS and iNOS were involved in pathagenesis of anovulatory functionalbleeding. While the expression of eNOS and iNOS were possibly regulated by estrogen orprogestin.