Detection And Clinical Significance Of Rotavirus Genome And IL-10 In The Blood Of Children With Rotavirus Diarrhea

Objective : To investigate the association of RV RNA with extraintestinal manifestations and to study the level of IL-10 and its relationship to the severity of illness in children with rotavirus diarrhea by measuring peripherial blood.Methods : The rotavirus RNA in the plasma samples and peripherial blood mononuclear cells (PBMCs) of 30 patients with rotavirus diarrhea were measured by two kinds of reverse transcriptase polymerase chain reaction (RT-PCR) tests . The levels of IL-10 in the prasma samples of 30 patients and 20 controls were tested by enzyme-linked immunosorbent assay (ELISA) . The positive PCR product was tested by agarose gel electrophoresis and was confirmed by analysing amino acid sequence. According to their severity of diarrhea , the patients with rotavirus diarrhea were divided into three groups , namely the gentle , moderate and serious groups .Results: One patient was found to be positive for RV RNA in the PBMCs and 30 patients were negative for RV RNA in the plasma. There were nodifference between the results of two kinds of RTE-PCR tests. Thepositive PCR product was confirmed as part of huraa?RV VP7 bytesting amino acid sequence . Identities of 94% with serotype G1 and75% with G9 were noted on amino acid sequences. Extraintestinalsymptoms were found in the positive case . Plasma concentration of IL-10 in the patients was higher than that in the control group( P<0.01) .Compared with gentle group , moderate and serious groups had higherlevels of IL-10 ( P<0.01). There was mild positive correlation betweenplasma concentration of IL-10 and total morbidity score ( P<0.05).Conclusion: RV RNA can be detected in the blood of children with rotavirusdiarrhea. Rotavirus may be transmitted by the PBMCs outside theintestinal tract and induce extraintestinal injury . IL-10 was probablyinvolved in pathogenesis of rotavirus diarrhea by serving as animportant inflammatory meditor and the level of plasma concentrationof IL-10 may be correlated to the severity of rotavirus diarrhea.