Study On The Nosogenesis Of Rotavirus Extraintestinal Infection

Rotaviruses (RV) not only cause acute gastroenteritis in infants and young children but also lead to extraintestinal infection. More and more attentions are paid to this. Although, there are few studies on the nosogenesis of it. The diffuse target organs and ways, the triggering factors are remained unknown yet. This study is to discuss them by the clinical studies and animal experiments. 1. On the clinical studies, we used the nested reverse transcription polymerase chain reaction (nRT-PCR) to test the RV viremia. The results showed that the morbidity of viremia in RV infected children is 16.6% in outpatients with common diarrhea and 20% in inpatients with severer symptoms. And they were found in the first day to sixth day in the diarrhea course, mostly in the second to fifth day. It is postulated that the viremia is the important factor of RV diffusing, which disappears after the diarrhea recovery. PBMC and the plasma can both carry the RV independently, and it is more common in the former. 2. Detected the trace elements of the RV infected children and found that most of the patients with viremia are disorder in trace elements. 3. Detected the TNFc~ and IL? by ELISA and the results showed that the lever of the TNFa was lower in the children with RV viremia than that of the ones without RV viremia, which suggests that there may be some association between viremia and the lever of TNFc~. 4. Abstracted the human RV strain from the fresh stools of RV infected children to inoculate the immunodeficiency and intestinal mixed infected mice, and the other kind of animal selected in this experiments is the newborn mice. Through them, we inoculated RV by the methods of taking orally and intraperitoneally. 5. After inoculation, there were different degree of damages in the liver, kidney, heart and small intestine by means of microscope and electron microscope (EM), and the RV double-stranded RNA could be found in the kidney and small intestine by in situ hybridization, and in liver, heart -5- and pancreas by in situ PCR. These results suggested that human RV can diffuse to the kidney, liver, heart and pancreas in the infected mice, the immunodeficiency, intestinal mixed infection and young ages are the triggering factors of RV diffuse outside the intestinal. The RV inoculated intraperitoneally is easy to diffuse than given orally. The intestinal barrier can protect the host from RV diffusing.