The Use Of CHA/bFGF In The Repair Of Segmental Radius Defects In Rabbits

OBJECTIVE To investigate the efficacy of combined use of basic fibroblast growth factor(bFGF)--coralline hydroxyapatite(CHA) in repairing segmental bony defects. METHODS CHA/bFGF composite was prepared and had been implanted in the comm continuous defects in ulna of adult rabbits , while CHA, autograft implantation or no graft were used as the controls . The animals were sacrificed 2,4,8and 12 weeks after operation , the bone repair process was investigated using physically , roentgenogram, histological observation and biology mechanics detection. RESULTS The vascularization in CHA/bFGF transplanted applied areas was better than that in CHA, autograft implantation or the control group 2,4 and 8 weeks after operation . Histological and roentgenogram examination showed that CHA/bFGF composite was implanted were repaired more rapidly than those with CHA or autograft implantation and in thecontrol group was filled with fibrous and muscular tissues. Biologic mechanics showed that on the CHA/bFGF side the callus was growing more quickly and vigorously , the bone density was higher and the antibending force was stronger than autograft implantation after 12 weeks. CONCLUSION CHA/bFGF can efficiently repair bony defect, and the capability of CHA/bFGF is the best of all by the way of satisfactory osteoconduction , osteoinduction . CHA/bFGF composite may be feasible for diaphyseal defect restoration , and the combination of CHA and bFGF is synergic in inducing bone formation .