Comparative Study Of Four Different Methords For Making Forelimb Lymphedema Models In Rabbits

Objective To compare four different methods of making forelimb lymphedema models in New Zealand rabbits. Methods In 32 New Zealand rabbits, we make forelimb lymphedema models by either axillary nodal/lymphatic microsurgical ablation(S)(guided by visual blue dye lymphography) or limited field-axillary irradiation(R) alone (2000cGy) or combined R followed by S or S followed by R. Observations were made for 18 weeks. Forelimb circumferences and volumes were determined serially and the findings compared with similar measurements in the contralateral non-manipulated limb.In randomly selected rabbits from R+S or S+R group, lymphatic drainage was assessed by motive lymphography, soft tissue swelling by magnetic resonance imaging(MRI), pathological changes of lymphedema were measured histochemically. Results R+S or S+R induced moderate to severe sustained limb lymphedema, remarkablely superior to S or R alonewhich produced only no or transient limb edema(P<0.01) . S+R was superior to R+S (P<0.01).Increased limb volume, impared lymphatic drainage(SPECT) , subcutaneous fluid accumulation (MRI) and obviously pathological changes of lymph- edema can be observed in R+S or S+R in 18th weeks . Conclusion R+S or S+R has the following advantages compared with S or R alone:early appearing, stable clinical condition , successful reproduction , similar colony of secondary lymphedema after operation and radiotherapy for cancer.It is a good method for making chronic limb lymphedema and should thereby facilitate standardized testing of therapeutic/preventive protocols and basic research into lymphatic dynamics in secondary lymphedema.