Mechanism Of Low Chronic Hepatitis B Cellular Immunity And Study On Its Prevention And Treatment

Objective To study mechanism of low chronic hepatitis B cellular immunity and the effect of Chinese drug DD on its prevention and treatment.Methods1. Animal experiment Wistar rats were devided into 5 groups in random: control group, chronic hepatopathy group (CH), CH+DD group, CH+L-Arg group and CH+L-NNA group. The control group was fed with general diet. In CH, CH+DD, CH+L-Arg and CH+L-NNA groups, the chronic hepatopathy model of rats established by complex pathogens was introgastric-infused with 0. 9% salt (1.5ml /d), DD(1.5ml/d), L-Arg(100ml/kg. d) , L-NNA (20mg/kg. d) respectively. At the end of the forth week, the level of plasma ET, NO, Il-2 and T lymphocyte subpopulation were detected. Left lobe of liver was fixed in 10% formaldehyde,than embedelinged in paraffin, at last observed histological change with optical microscope.2. Clinical experiment Select 20 healthful persons in random in control group. 39 chronic hepatitis B patients were divided into general therapy group(GT) and GT+DD group. In GT, patients were treated with general medicines. In GT+DD group, patients were treated with DD for a month on the base of GT. At the end of one month, the level of plasma ET, NO, Il-2, T lymphocyte sub-population and HBV-DNA were detected.Results1. Animal experimentThere was significance difference between control group and CH (p<0.01), and so were CH+DD and CH, CH+DD and CH+L-Arg. There was no significance difference between CH+DD and CH+L-NNA (p>0.05). ET was positively correlated with NO and CD8+ respectively (r=0.827, p<0.01; r=0.469, p<0.05); ET was negatively correlated with Il-2, CD4+ and CD4+/CD8+ ( r=-0. 969, p<0. 05; r=-0. 683, p<0. 05; r=-0. 829, p<0.05 ).2. Clinical experimentThe level of plasma ET, NO, CD8+ and HBV-DNA in GT and GT+DD were higher than that of control group(p<0.01), but the level of plasma IL-2, CD4+ and CD4+/CD8+ in GT and GT+DDwere lower than that of control group (p<0.01). The level of plasma ET, NO, CD8+and HBV-DNA in GT and GT+DD decreased after treatment (p<0. 01) ; the level of plasma IL-2, CD4+ and CD4+/CD8+ in GT and GT+DD increased after treatment (p<0.01). There was significance difference between GT and GT+DD (p<0.01). ET was positively correlated with NO and CD8+ respectively (r=0. 497, p<0.01 ; r=0. 178, p<0. 05); ET was negative cor related with IL-2,CD4+ and CD4YCD8+ (r=-0.503, p<0.05; r=-0.427, p<0.01; r=-0. 508, p<0. 01).Results showed that cellular immunity of chronic hepatitis B patients was low, its mechanism was correlated with IETM and NO.ConclusionsThe level of plasma ET in chronic hepatitis B was high, which resulted in low cellular immunity, NO perhaps was one of mediums. DD can decrease the level of plasma ET and NO to modulate immunological function so that it is beneficial in the prevention and treatment of chronic hepatitis B.