Glutamic Acid Decarboxylase Delays Type 1 Diabetes In NOD Mice

Objective1. Immune medicament can be chosen.2. Different dose of GAD effects the incidence of diabetes in NOD mice. And the function can be discovered.3. GAD effects the incidence of diabetes in different week old NOD mice. And mechanism can be found.Methods1. Twenty NOD mice in three-week old were devided into 4 groups at random . Group 1 was injected GAD3mu ; group 2 was injected GAD3mu+IFA50ul ;group 3 was injected EFA50ul ;group 4 was injected PBSSOul as control group. Blood glucose were measured in every two weeks. At last group 2 was the lowest in the incidence of diabetes. Therefore GAD3mu+IFA50 l is as the observation in this experiment.2. Based on experiment 1 result ,GAD3mu+IFA50 l is the observation. Being found different dose of GAD effection the incidence of diabetes in NOD mice,40 mice were devided into 4 groups at random. In group 1 mice were injected GAD0.03mu+IFA50 l; in group 2 mice were injected GAD3mu+IFA50 l; in group 3 mice were injected GAD0.3mu+IFA50 l; group 4 is control group.Blood glucose and the serum leveles of IL-4 and y-IFN were measured in regulation time . At the age of 28 weeks , mice were killed and the subsets of T cell in spleen and in thymus were determined by two-clolor FACS analysis . Parcreatic histopathology studies were performed by H-E.3. Injection GAD3mu+IFA50 l in belley effect the incidence ofdiabetes in different week old NOD mice.40 mice that were three week old are randomly devided into 4groups.In group l,mice were injected in three week old ;in group 2, mice were injected in eight week old;in group3 ,mice were injected in twelve week; group 4 was control group.Blood glucose and the serum leveles of IL-4 and y-IFN were measured in regulation time . At the age of 28 weeks , mice were killed and the subsets of T cell in spleen and in thymus were determined by two-clolor FACS analysis . Parcreatic histopathology studies were performed by H-E. Results1. In GAD3mu+IFA50 l group,mice blood glucose balance . Therefore GAD3mu+IFA50ul is observation in thise experiment.2. In group 2 , mice that were injected GAD3mu+IFA50ul had kept balancein blood glucose. The serum levels of IL-4 were increased significantly ,and y-IFN was on the contray (p<0.01). The lymphocytic inflammation of pancreatic islets in group 2,and the insulitis score decreased markedly(p<0.01). A strong proliferative response of CD8+ subsets in splenocytes occurred in 2group, and CD4+/CD8+ ratio was lowest in the groups (p<0.01) .3. In group 1 blood glucose are normal level ,on the contrary other groups increased very rapidly .In group 1 mice IL-4 level in surms kept balance, and y-IFN level increased lowest in all groups(p<0.001). The insulitis score was the smallest(p<0.01). CD4+/CD8+ratio is lowest in all groups(p<0.01).ConclusionGAD3mu+IFA50ul prevents the onset of type 1 diabetes and reduces the risk of insulitis in female NOD mice at the age of three weeks.Its mechanisms seem to be related to the regulation ThO cell switch to the Th2 eel by cytokine such as IL-4 .