The Combined Effect And Antitumor Mechanism Of Genistein And 5-FU On Human Coloretcal Cancer Cell Lines Colo320 And SW480 In Vitro

OBJECTIVES: To study the combination effect and antitumor mechanism of Genistein and 5-FU on Human coloretcal cancer cell lines Colo320 and SW480 in vitro and its mechanism of action.METHODS: MTT assay was used to analyze the effect of Genistein and 5-FU alone or in combination on the growth of Colo320 and SW480 cells. Flow cytometry and immunocytochemical staining were used to observe the morphologic changes, cell cycle and expression of Bax, Bcl-2, mtant P53, VEGF of these cells.RESULTS:1.Alone or in combination Genistein and 5-FU could inhibit the growth of Colo320 and SW480 cells in a time and dose dependent fashion. The combination of genistein with 5-FU had synergistic effect on the Colo320 cell line when the inhibition rate exceed 78% and on the SW480 cell line when the inhibition rate exceed 93%.2.Colo320 and SW480 cells presented some morphologic features of apoptosis after treatment with Genistein and 5-FU for 72 hours.3.Genistein could arrest cell cycle of Colo320 and SW480 cells in Ga/M phase.4.Genistein and 5-FU could downregulate the expression levels of mtant P53, VEGF, Bcl-2, upregulate the expression levels of Bax.CONCLUSION:1.Alone or in combination Genistein and 5-FU could inhibit the growth of Colo320 and SW480 cells effectively .The combination of them showed synergistic or antagonistic effects at different concentration.2.Genistein can hardly change the distribution of cell cycle.3.Genistein may inhibit angiogenesis in colorectal carcinoma by reducing the expression levels of VEGF.4.Genistein can induce apoptosis in Colo320 and SW480 cells by reducing the expression levels of mtant P53. Bcl-2, upgrade the expression levels of Bax.