| Background and objectives: Neoplasm,shown much solicitude for its severe harm,has been investigating from the perspective of different fields and levels.However,there is still no approaches available so far.The phenomena that decidua cells in early pregnancy could inhibit not only trophoblast cells proliferation and invasion,but also the choriocarcinoma cells(BeWo cell) growth which was first confirmed by Lewis in the early 90,s. Investigaters also found the 57DR-NS cells isolated from human decidu could even induce apoptosis in some cancer cells. The factors responsible for these important regulatory process are unknown, but in vitro studies indicate some decidual cytokines and growth factors are posible candidates.It was found that the full term pregnancy decidua conditioned medium could also inhibit the invasion of trophoblast cells ,and more effective than early pregnancy decidua,s.The aim of this study was to investigate the effect of decidua-medium from human term pregnancy(DCM) on morphology ,proliferation and invasion in 9HTE,SKOV3,MDA-MB-231,HeLa,JAR,SMMC-7721,A549 and K562 cells.The mechanisms of them were also explored.Methods:Decidua obtained from healthy full-term pregnancy was performed to prepare for conditioned medium by tissue primary culture. Control experiments were done with fresh serum-free RPMI 1640 medium,and cell lines are 9HTE,SKOV3,MDA-MB-231,HeLa,JAR,SMMC-7721,A549 and K562. Light microscope,hematoxylin eosin staining (H.E),Wright staining and transmission electron microscope (TEM) were conducted to determine the effect of DCM on cells morphology. The effect of DCM on cells growth was determined by MTT.Flow cytometry was adopted to determine the cell cycle,and in situ hybridization and immunocytochemistry detect the expression of PCNAmRNA and cyclinD1. The investigation of invasion were determined by transwell insterts and RT-PCR which detected the expression of MMP-2,MMP-9 and TIMP-1mRNA.Results:Light microscope revealed that there were nearly no change in 9HTE,HeLa,T24,SMMC-7721,A549 cells treated by DCM, but the cellur density of the SKOV3 and MDA-MB-231 cells increased and they had more cells in division and proliferation than the control experiment. H.E staining and Wright staining showed that the treated JAR cells chromosome condensed and pyknosis.Meanwhile,K562 cells contraction and nuclei pyknosis,membran rupture,cytoplasm lysis. Under electron microscope, the ultrastructure of SKOV3 and MDA-MB-231 cells was normal,but karyokinesis significantly increased. However,in JAR cells,inequality of size of lipid drops and myeloid-like structure appeared in the cytoplasm,chromatin condensed,quite a few apoptosis bodies formed. In a few JAR cells,the mitochondrion,endoplasmic reticulum and cytoplasm swelling. The disintegration of nucleolic manifested by increasing number of nucleolar and apoptosis were detected in K562 cells. MTT displayed that DCM treatment accelerated SKOV3 and MDA-MB-231 cells proliferation and inhibited K562 cells growth in a dose-dependence manner (p<0.05),but had no effect on the other cells,including 9HTE,HeLa,T24,SMMC-7721 and A549 cells(p>0.05). Flow cytometry showed that after the treatment,SKOV3 and MDA-MB-231 cells arrested at G0-G1 phase (p<0.05),while a large number of K562 cells entered S and M phases (p<0.05),but there were no change in 9HTE,HeLa,T24,SMMC-7721 and A549 cells(p>0.05) after the treatment. The expression of PCNAmRNA and cyclinD1 showed that they were expressed in cytoplasm and nuclei respectively,and both of them expressed in the treated SKOV3 and MDA-MB-231 cells were much more than the control experiment(p<0.05).In the experiment of transwell inserts,it was found that the mobility of the treated K562 cells dropped down(P<0.05),while the treated MDA-MB-231 cells had the opposite effect(P<0.05), there were no change in 9HTE,HeLa,T24,SMMC-7721 and A549 cells(p>0.05). RT-PCR showed that the expression of MMP-2 and MMP-9mRNA were down-re...
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