The Changes Of Nuclear Matrix Protein In Human Colon Carcinoma

Objective: The nuclear matrix is a structure obtained after extraction of nucleic acids, the soluble histones and phospholipids with detergents, high ionic strength salt, and DNases. More than 98 percent of residual component of the nuclear is protein, called as nuclear matrix proteins (NMPs). The nuclear matrix proteins play an important role in the regulation of intranuclear processes such as DNA replication, gene transcription, and RNA processing. Studies have shown that nuclear matrix proteins(NMPs)are both tissue and cell type specific.The objective of this study was to identify the existence of the specific NMP,which may serve as tumor markers, in the human normal colon tissue and colon carcinoma,try to study the association between NMPs and colon carcinoma .Methods: High resolution two-dimensional gel electrophoresis and silver staining were used to identify the NMPs in 12 matched human colon carcinomas and adjacent normal samples. MMP-2 and CB were detected by immunohistochemical SP method. Result: Analysis of multiple gels for each sample revealed 5 proteins identified with C1—C5 presented in tumor samples that were not presented in the matched normal colon tissues, and 3 proteins identified with N1—N3 presented in normal colon tissues that were not presented in the colon carcinomas. There was different NMPs in different types of colon carcinoma. Nuclear matrix protein N4 presented in 9 moderate—well differentiational colon carcinomas, but was not present in 3 poor differentiational colon carcinomas. The result of immunohistochemistry showed that the expression of MMP-2 and CB in poor differentiational colon carcinoma which absence of expression of NMP N4 was strongly positive,and stronger than that in the moderate—well differentiational colon carcinomas which expressed NMP N4. Conclusion: These data suggest that there are colon carcinoma- specific NMPs. There are different NMPs in different types of colon carcinomas. Colon carcinoma-specific NMPs may be serve as the tumor markers of colon carcinoma. The changes of NMPs maybe play a role in colon carcinoma progression.