| OBJECTIVE: To study the correlation between the expression and clinical significance of tumor suppressor gene PTEN protein in primary gastric carcinoma and its possible role in tumorigenesis and development, so as to provide theoretical basis for diagnose, therapy and prognosis.METHODS: 3 normal gastric samples, 55 cases of gastric carcinoma and their corresponding paracancerous tissues and distant-tumor tissues were used for detection. Immunohistochemical staining for PTEN protein was performed with SP method on frozen sections. The results were associated with clinical pathological data were analyzed.RESULTS: (1) The cellular localization of PTEN was found in the cytoplasm. PTEN was expressed in all of normal gastric tissues and it' s protein expression level in distant-tumor tissues and in paracancerous tissues was significantly higher than that in gastric carcinoma (P <0. 05) . (2) The expression of PTEN protein was no correlation with sex, age, tumor size, location, pathological type, distant-organ metastasis, Correa' s and Borrmann' s classification. (3) The expression of PTEN protein was significantly lower in gastric carcinoma with lymph node metastasis than without (P<0. 05) . (4) The expression of PTEN protein was positively related to histological differentiated grade (rs=0. 362) and negatively related to invasion depth (rs=-0. 343) and clinical TNM stage (rs=-0. 375) .CONCLUSION: (1) PTEN gene was associated with carcinogenesis and progression of gastric carcinoma. (2) It was suggested that analyzing the expression of PTEN protein should be a new adjuvant diagnosis index for judging tumor pathological biological behaviors and prognosis. (3) There were adjacent tumor tissues with genetic alterations while they were normal in morphology.
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