The Expression Of P53 Protein And Its Relationship With Clinicopathological Feature And AFP, HBV Infection In Hepatocellular Carcinoma

Objective To investigate the expression of p53 protein inhepatocellular carcinoma (HCC) and benign hepatic tumor and itsrelationship with clinicopathological feature and prognosis of HCC andalpha-fetopritein (AFP) and hepatitis B virus (HBV) infection in HCC. Methods The expression of p53 protein in 70 specimens of HCC and 20specimens of benign hepatic tumor from Guangxi was detected byimmunohistochemical S-P method. And it's relationship with clinical andpathology grade of HCC, portal venous tumor emboli, AFP and HBVinfection was analysed. Results The expression rate of p53 protein was 44.29%(31/70) in HCCand 0%(0/20) in benign hepatic tumor respectively and there was asignificant difference between them (P<0.01). There was no significantrelation between the expression of p53 and patients' sex, age, clinical stage,tumor size, and cirrhosis of the HCC (P>0.05), but there was a significantrelation between the expression of p53 and Edmondson's histopathologicalclassification (P<0.05). A significant difference in the expression rate of p53between serum AFP-positive group and AFP-negative HCC group wasobserved (54.35%, 25/46 vs 25.00%,6/24ï¼›P<0.05). A similar result was alsoobserved in HbsAg-positive and HbsAg-negative HCC group (58.14%,25/43 vs 22.22%,6/27ï¼›P<0.01). The p53 protein was expressed morefrequently in PHCC with incomplete capsule or venous tumor emboli than inthose with complete capsule (60.71%, 17/28 vs 33.33%, 14/42; P<0.05) or 3no venous tumor emboli (100%, 6/6 vs 39.06%, 25/64; p<0.01). Meanwhile,the postoperative 2-year survival rate (35.48%, 11/31) of patients with HCCwith p53 gene expression was significantly lower than those without p53gene expression (76.92%, 30/39) (P<0.01). Conclusion 1. p53 gene mutation, which leads to over-expression ofp53, is a common molecular structure changes in HCC. It is closely relatedto histopathological classification and invasiveness of human HCC and itcan be used as a valuable index in evaluation the prognosis of HCC. p53may be an ideal tumor targeted gene in gene therapy for HCC. 2. Theexpression of p53 protein in HCC is closely related to serum AFP level,which indicates that there may be a mutual active mechanism betweenexpression of p53 and AFP. 3. There is a close correlation between p53gene expression and HBV infection in HCC, suggesting that HBV infectionmay lead to hepatocarcinogenesis by stimulating the mutation of p53 gene.