| ObjectiveAcute pancreatitis (AP) is a self - digested chemical inflammation by auto -secretted digestive enzymes. From pathohistology and clinical manifestation, it is divided into two types; acute edematous pancreatitis and acute hemonecro-tizing pancreatitis. Acute hemoneerotizing pancreatitis develops rapidly and has a high mortality, its development mechanics have not yet been completely elucidated . A research considered the level of cytokines in blood was correlated with pathological type of acute pancreatitis, but not confirmed. Large dosage injection of L - arginine may injure pancreas and lead to necrosis, we can develop an animal model of acute pancreatitis in this study based on this principle, notice the pathological changes of 1d 3d 7d 10d 14d in rats after injecting arginine. Based on the effect of transforming growth factor - β1 and Matrix Metalloprotein-ase - 2 in cellular growth and tissue remodelling. Detest the immunoreactivity of two cytokines of all periods through immunohistochemistry, determine the role of regeneration after acute necrotizing pancreatitis, confirms the biological properties of two cytokines, these will help us to grasp the principle of acute pancreatitis, especially the regeneration after acute necrotizing pancreatitis, helpful to clinical services.MethodsSixty male Wistar rats , weighing 180 -220g, randomly grouped, 10 in one group. Killed by decapitated in all periods after injecting arginine, removedeach pancreas and fixed overnight in 10% formalin, embedded in paraffin, and deparaffinized by the standard procedure. Immunohistochemistry is completed by routine SP methods. Slides are processed with polyamine, and the process is completed after hot repairement of antigen with EDTA.Results1. Immunohistochemical staining of TGF - β1 began to increase on day 1 after injecting arginine , obvious on day 3, and reached a peak value on day 7. later, began to decrease gradually with the regeneration of necrotizing pancreatic cells, on day 14, similar to the level of control group.2. Immunohistochemical staining of MMP2 is expressed in all groups, higher in control group, and began to decrease on day 1 after injecting arginine. later began to rise, reached a peak level on day 7, later to decrease, on day 14, to a higher level after regeneration completely.3. Use x2 test of two samples, all values are contrasted with the value of control group, on day 3,7 after injecting arginine, TGF - β1 p < 0. 05, on day 1 after injecting arginine, MMP2 p < 0.05 , the variances are obvious, and have statistic significances.4. Others p > 0. 05, the variances are not obvious, no statistic significances.DiscusionAlthough acute hemonecrotizing pancreatitis is more severe, once the primary causes or factors are eliminated, the structure and functions of the exocrine pancreas are often followed by full recovery. TGF-β1, is known as a group of cellular growth factors with many biological properties, such as inducing transforming , differentiating and inhibiting the proliferation of cells, it' s inhibiting the proliferation of cells is used to the research of tumors. TGF-β1 is not only the ECM modulator of pancreatic remodelling, but also correlated with the acute pancreatitis. Moreover, in hemonecrotizing pancreatitis, TGF - β1 may inducehepatocytes to atrophy in pancreatic ascites. TGF - β1 is a protein made of two polypeptide chains, a multifunctional cytokine group , having 5 subtypes and have been cloned, TGF - β1 may lie in all tissues, abundant in bone, lung, kidney and placenta, mainly originated in lymphatic cells, thrombocytes, mono-cytes/ Macrophages. TGF - p, can modulate cells to complete many functions, a bidual modulator of cellular growth, modulate the growth and differentiation of cells, induce matrix to appear and atrophy. In the course of the development of embryo, TGF - β1 play an important role in the formation of model and specific differentiation of tissue. In adults, it is concerned with the repairement o...
|
PDF Full Text Request