The Study On The Expression Of The Granulocyte Colony-stimulating Factor Receptor And The Granulocyte-macrophage Colony-stimulating Factor Receptor On The Cells In The Blood Of The Systemic Lupus Erythematosus Patients

ObjectiveSystemic lupus erythematosis (SLE) is an autoimmune disease,the exactly pathogenesis of SLE is not clear, the commonly opinion is that the environments act on body with certain heredity factors.In the research on genetic factors, people found that polymorphism of some genes of cytokines and cytokine receptors may play a role in SLE. We have found that the G - CSF gene and the G - CSFR gene may play a role in susceptibility to SLE. Based on those above, we detect G - CSFR and GM - CSFR on the surface of target cells in the patients with SLE through flow cytometer in order to explore the association between G - CSFR,GM - CSFR and SLE.Material and Method1. Material: flow cytometer; R - Phycoerythrin ( R - PE) - conjugated mouse anti - human monoclonal antibody ( CD -114 , CD -116).2. We studied 39 SLE patients who met the 1982 revised criteria of the A-merican Rheumatism Association for systemic lupus erythematosis. And there were still 23 patients with rheumatoid arthritis involved in the study.3. Clinical and laboratory data came from our case records and results from laboratory examination.4. Method: We collected the blood samples, made response of antigen and antibody, then made the sample into fluid consist of separated cells. Using flowcytometer, recorded the mean fluorescence intensity of G - CSFR and GM - CS-FR on the surface of target cells. All the collection was analyzed by Cell Ques Plot software. Then performed statistic analysis for experimental results.Results1. The mean fluorescence intensity of GM - CSFR on the surface of mono-cyte in total SUE case group and RA case group has significant statistic difference compared with normal control group (P=0.001, P=0.002 respectively) ; but, there is no significant difference between SUE and RA (P >0.05).2. The mean fluorescence intensity of GM - CSFR on the surface of mono-cyte in SUE subsections all have significant statistic differences compared with normal control group ( active P = 0. 001; non - active P = 0. 045; non - treated P =0.001; treated P =0. 004). But, there is no significant difference between SLE subsections and RA. And there is no significant difference between active and non - active groups, treated and non - treated groups.3. The mean fluorescence intensity of G - CSFR and GM - CSFR on the surface of neutrophil in total SLE case group , SLE subsections has no significant statistic difference compared with normal control group (P>0.05).4. The mean fluorescence intensity of GM - CSFR on the surface of mono-cyte in SLE kidney related group has no significant statistic differences compared with SLE kidney unrelated group (P>0.05).Conclusion1. The mean fluorescence intensity of GM - CSFR on the surface of mono-cyte in SLE case group has significant statistic difference compared with normal control group, there is no significant difference between active and non - active groups, treated and non - treated groups of SLE, and there is no relationship between GM - CSFR with LN.2. The mean fluorescence intensity of GM - CSFR on the surface of mono-cyte in RA case group has significant statistic difference compared with normalcontrol group , there is no significant difference between SLE and RA.3. There is no relationship between G - CSFR and SLE,LN.We found that there is relationship between GM - CSFR and autoimmune disease including SLE.