| ObjectiveHuman brain gliomas are the most common malignant tumors of central nervous system, rich in vessels, aggressive and easy to recur. These biological behaviors often make clinical management fail to work. Many procedures are involved in the process of invasion of brain gliomas. Tumor cells adhere to extracellular matrix through the membrane receptor. Local matrix is degraded by protease secreted by tumor cells. Tumor cells move towards the region in which the ECM is degraded. The degradation of ECM is considered as a token of invasion of tumors. Of the proteases, MMP-9, which plays a significant role during the course of tumor aggression, is the most important. It can be inhibited specifically by TIMP. Rapid proliferation of tumor cells depend on nutrition supplied by vessels. In addition, the high permeability of new vessels facilitated the spread of tumor cells. bFGF significantly stimulates angiogenesis. It is related with the growth and extension of tumors. Its inhibition slows down the growth of tumor cells. Therefore, through the determination of MMP-9 and bFGF expression in normal brain tissues, low - grade gliomas and high - grade gliomas, we can understand the roles they may play in the development of gliomas. This may help to find a new way for the treatment of gliomas.MethodsIn this experiment, we utilized immunohistochemistry technique to examine the expression of MMP-9 and bFGF in normal brain tissues, low - grade gliomas and high - grade gliomas respectively.Result determining; the brownish staining of cytoplasm, vascular basement membrane and endothelial cells is regarded as MMP-9 positive. The positive ex-pression of bFGF is judged by brownish granules showing in cytoplasm. We count the number of positive cells in 5 randomly selected visual fields each slide. Then, we divide the cases into two groups according to the ratio of positive cells. The cases in which the ratio is less than 10% belong to the negative group. Those in which the ratio is more than 10% belong to the positive group. Statistical methods: the positive expression among three groups and the correlation between them( MMP-9 and bFGF) are analyzed by the \ test. P <0.05 is considered significant .Results1. The expression of the MMP-9 MMP-9 is expressed in cytoplasm of glioma and vascular endothelial cells. It is also seen in vascular basement membrane. There are no expression in normal brain tissues. Average positive ratio of MMP-9 in all glioma sample tissues is 63. 5%. In low - grade gliomas ,the positive ratio is 37.0% , while it is 92.0% in high - grade gliomas. Analysis by \2 test show there is no significant difference between low - grade gliomas and normal brain tissuses( P > 0. 05 ). Hie comparison of low - grade gliomas and high -grade gliomas is significant( P <0.05 ).2. The expression of the bFGF bFGF is expressed in cytoplasm. In normal brain tissuses it is negtive. The ratio of positive cells in low - grade gliomas is 48.1% , while in high - grade gliomas it increases to 80%. Significant differences exist among three groups ( P < 0.05 ).3. The correlation between MMP-9 and bFGF MMP-9 and bFGF are both expressed in 24 gliomas, MMP-9 is expressed in 9 gliomas alone , and bFGF is expressed in 9 gliomas alone too,neither MMP-9 nor bFGF is expressed in 10 gliomas'tissues. Significant difference exist between two groups (P<0.05).ConclusionThe expression level of MMP-9 and bFGF increase in parallel with the grade of gliomas. This indicates MMP-9 and bFGF may be associated with theaggressive behavior of human gliomas. There are correlation between MMP-9 and bFGF in the gliomas'growth.
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