The Influences Of Dendritic Cell In Lymph Nodes On Prognosis Of Kideny Neoplasm

OBJIECTIVE: The objective of this study was to investigate the correlation between dendritic cells in tumor microenvironment and tumor draining lymph nodes and prognosis of advanced kidney neoplasm. It was probed into for the significance of the marker for marking dendritic cells and importance was attached to immature dendritic cells for the prognosis of tumor on basis of the result of the experiment. METHODS: 59 patients with advanced kidney neoplasm whose nephrectomy were more than 5 years and the lymph node specimens were kept were selected, and their tumor and lymph node specimens were re-preparation. Density and distribution of dendritic cells was analyzed by immunohistochemistry performed by the SABS method using antibody against GD1 a in tumors and antibody against S-100, GD1 a in lymph nodes for each patients. The prognostic significance of dendritic cells infiltration in tumor microenvironment and tumor draining lymph nodes were evaluated respectively.RESULTS: CD1 a DCs infiltration in tumor microenvironment was positively correlated with the prognosis. CD1 a +DCs infiltration is45.3 13.1 / mm2 in the group whose patients is not metastatic or recurrent at least 5 years after nephrectomy; GDI a +DCs infiltration is 29.3 10.3 / mm2 in the group whose patients is either metastatic or recurrent in 5 years after nephrectomy. There was a significant difference between groups, (t=5.16, P<0.01). S-100+, GD1 a +DCs infiltration in tumor draining lymph nodes was positively correlated with the prognosis. S-100+DCs infiltration is 68.79 21.59/mm2 in the group whose patients is not metastatic or recurrent at least 5 years after nephrectomy; S-100+DCs infiltration is 46.04+24.19/mm2 in the group whose patients is either metastatic or recurrent in 5 years after nephrectomy. There was a significant difference between groups. (t=-6.20, P<0.01). Low S-100+DCs infiltration ( <57/mm2) is 38.83 12.70/mm2 (worse prognosis: 65; better prognosis: 22); high S-100+DCs infiltration(>57/mm2) is 80.78 17.86/mm2 (worse prognosis: 15; better prognosis: 54). There was a significant difference between groups in the constitute( x 2 = 43.22 , P<0.01). GD1 a +DCs infiltration is 35.1 13.0/mm2 in the group whose patients is not metastatic or recurrent at least 5 years after nephrectomy; GDI a +DCs infiltration is 25.9 9.6/mm2 in the group whose patients is either metastatic or recurrent in 5 years after nephrectomy. There was a significant difference between groups. (f=-5.06, P<0.01). Low GDI a +DCs infiltration(<30/mm2) is 21.13 5.29/mm2 (worse prognosis: 61; better prognosis: 27); high GDI a +DCs infiltration(>57/mm2) is 42.33 7.38/mm2 (worse prognosis: 19; better prognosis: 49). There was a significant difference between groups in the constitute( x 2=26.29,P<0.01).CONCLUSIONS: DCs in tumor draining lymph nodes play an important role in immunological defense mechanisms of the host against kidney neoplasm. It is controversial that S-100 mABs for marking DCs is applied in anticipating the prognosis. It may attach importance to immature DCs (GD1α +DCs).