Study On The Expression Of Dendritic Cells Surface Antigen CD11c, CD80, CD86Intestinal Mucosa In Patients With IBS

Objective To study the expression of dendritic cells（DC）surface antigen CD11c，costimulatory molecules（CD80、CD86）intestinal mucosa in patients with irritable bowelsyndrome（IBS），to understand the distribution and maturity of DC，to understand thechanges of IBS patients in the intestinal mucosal immune function，and to explore theirroles in the pathogenesis of IBS.Methods Collected detailed history and executed physical examination of therelevant systems，and then completed the necessary auxiliary examination（blood routine、urine routine、stool routine、blood biochemistry、abdominal ultrasound），according tothe inclusion criteria selected40cases of IBS patients as IBS group. Another40cases forthe same period subjects were healthy volunteers as normal control group. Firstly，observed the whole colon mucosa situation by colonoscopy after the full bowelcleansing；Secondly， mucosal specimens were harvested from sigmoid colon bycolonscopy in40patients with IBS and40healthy controls，while histologicaldiversification was evaluated by histological examination. The expression of intestinalsurface molecules CD11c，costimulatory molecules（CD80、CD86）was detected byimmunohistochemistry.Result Histopathological analysis showed that IBS patients and control subjectswere intact intestinal epithelium，glands were normal，no interstitial edema and neutrophilinfiltration of acute inflammatory changes. Compared with control group，the expressionof CD11c、CD80and CD86in sigmoid colon of IBS group were increased（P＜0.05）. Conclusions IBS patients possessed intestinal immune activation. Intestinaldendritic cells may be involved in the immunological pathogenesis of IBS，the exactmechanism needs further study.