| Objective:To study the relationship between the effect of the radiation of HPM and the time of radiation or average power density of HPM, and the regulation of the apoptosis caused by the HPM radiation. Further more, To discuss the mechanism of the apoptosis of the germ cell induced by the HPM radiation at the protein level.Methods:1 The fixed experimental rats were radiated with S wave band 10 mW/cm2, 20mW/cm2, 40mW/cm2, 80mW/cm2 HPM for lminute,5minutes, lOminutes and 20 minutes respectively or sham treatment to build the model of the animal. Testicular or ovarian samples were taken 6h, 24h, 48h, 72h and 5d after radiation , separately fixed with 4% buffered formaldehyde or 3% buffered glutaraldehyde and embedded in paraffin or tetroxide osmate.2 The morphology and the ultrastructure of the testis or ovary were observed by light microscope or transmission electron microscope respectively.3 Testicular or ovarian germ cell apoptosis was detected by in situ terminal deoxymudeotityl transferase mediated dUTP nick end labeling(TUNEL).4 At the end of the process, immunohistochemical S-P staining was performed to test the expressions of Bcl-2 protein and C-myc protein in germ cell of the testis or ovary in rats. Results:1 With light microscope, vascular congestion and interstitial edema , slightly inflamatory cell infiltration were observed in the 20 mW/cm2, 5 min group , in addition the germ cells of germinal epithelium irregulation and deformation in the spermatogenesis were also observed in the 20 mW/cm2,10 minutes experimental groups; Partly convoluted seminiferoustub -ules androgone necroses were also observed in 20mW/cm2,20min and 40mW/cm2,lmin group. In 40mW/cm2,5min group, the deadly damage reached the limit, there were nealy no cells in partly convoluted seminiferous tubules. With the transmission electron microscope, slight mitochondrion edema and endoplasm reticulum dilation, spermatogonium degeneration were observed in the 20mW/cm , 5 min group; mitochondrion vacuolation were found in 20mW/cm ,10 minutes experimental groups; a swollen spermato -gonia with rarefaction of nuclear chromosome and obscurity of mitochondria structure with reduplication of splitting of basal membrane were observed in the 20 mW/cm2, 20min, Interstitial cells degeneration, apoptosis and necrosis were also found . But no change was found in contral group.2 From 6h experimental groups , the number of apoptosis cells was remarkably larger than the control's till 48h after radiation(P<0.01)in 10 mW/cm2,5min groups and 20 mW/cm2,5min groups. Especially the 10 mW/cm2 ,5min,6 h experimental group reaching the peak (161.27 + 5.9) IS convoluted seminiferous tubules. The change of the other experimental groups was the same as that of the control (P>0.05).3 The expression of the Bcl-2 protein and C-myc protein were (+ + +)degree in 24 groups after radiation by 10 mW/cm2,20 mW/cm2 HPM for 5min and 10min.In addition, the expression of the Bcl-2 protein was (+ + ) degree and that of the C-myc protein was( - )degree in 10mW/cm2,10min,48h experimental groups; The expression of Bcl-2 protein was ( + + ) degree andthat of C-myc protein was ( + ) degree in 20mW/cm2,5min, 6h experimental groups . No change was found in contral group and the other experimental groups.4 With light microscope, vascular congestion , interstitial edema and slight inflamatory cell infiltration were observed in the 40 mW/cm2,5 min group, in addition granular cells irregulation were also observed in follicle in the 40mW/cm2,10 minutes experimental groups; granular cells degeneration, lutein cell apoptosis and unilunar follicle, primary follicle and secondary follicle atresia were also observed in 40mW/cm2,20min and 80mW/cm2,lmin group, 5min groups and 10min groups. In 80 mW/cm2,20min group, partly granular cells, Interstitial cells necrosis and tissue haemorrhage were found. With the transmission electron microscope, slight mitochondrion edema and endoplasm reticulum dilation were observed in...
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