The Expression Of HIF-1α In Placenta Of Pregnancy Induced Hypertension (PIH)

Pregnancy-induced hypertension (PIH) is a pregnancy specific disease that characterizes as hypertension, proteinuria and edema. Severe PIH can be complicated with intra-uterine fetus death and maternal death due to cerebral haemorrhage and renal and heptic failure. PIH can spontaneously relieve its symptoms and signs after the delivery of placenta, indicating that it is truly a 'placental disease' . The theory of ischemic and hypoxemic placenta has been generally accepted in numerous etiological investigations. HIF-1 is the transcription factor which widely exists in mammaland human body under the hypoxemic condition. We investigated the expression of HIF-1 in PIH placentas to try to find out the role and significance of HIF-1 in the process of PIH.ObjectiveTo investigate the expression of Hypoxia inducible factor-1 a (HIF-1 a )in placentas of pregnancy induced hypertension (PIH) . Methods1. RT-PCR was used to detect the expression of HIF-1 a mRNA in placentas of PIH pregnancies and normal pregnancies.2. Immunohitochemistry was carried out to investigate for HIF-1 a protein expression using placentas tissue microarray.Results1. The level of HIF-1 a mRNA between the PIH group and the control group (0.957 + 0.369 vs 0.954 + 0.198, P>0.05) was not statistically different.2. There was significant difference in the positive and slight-mid-positive spot proportion of HIF-1 a protein between the PIH group (63.1%; 23.3%) and the control group (20.9%; 7.0%) (P<0.01) .Conclusions1. HIF-1 a regulates the pathological and physiological progress of PIH at protein level rather than at the transcription level.2. Tissue microarray has the superiority of efficiency, economy and consistency which can be used to quickly test the level of the protein coding by the aimed gene. Our research has provided a new idea for investigation of the related gene about PIH.