Study Of Relationship Between The Infection Of Licobacter And Hepatocellular Carcinomas By Detecting 16SrRNA

Objective: To determine whether there was the relationship between theinfection of Helicobacter 16SrDNA and hepatocellular carcinoma (HCC) with the technique of molecular biology. To provide new way for the reason and mechanism, moreover to give new path to clinical diagnosis and prevention treatment of HCC.Method: (1)With DNAstar to unload all of the 16SrRNA sequences ofhelicobcter , while comparing with the universally conserved domains of 16SrRNA of E. Coli, S. pyogened and P. Penumotropica , a pair of specific Helicobacter primers and reaction factors for PCR amplication and Southern hybridization were planned. (2)Select 15 HCC patients for lab group, and 15 other patients respectively such as gastric cancer non-HCC carcinoma of colon and rectal and leiomyoma of uterus for contrast groups . Some cancer tissue of hepatic and gastric cancer groups were extracted for DNA preparing for PCR and Southern hybridization , the other and the rest contrast groupswere for pathologic slides and for in situ hybridization. ㏕he probe of Helicobacter designed by our lab and the probes of Hh Hp Hf designed by Battle were labelled with DIG to detect 16SrRNA-mRNA .(4) Finally, all the positive of HCC were sequenced and the type of helicobcter were analyzed.Result: (1)We detected 15 HCC patients by PCR and the positive (9 of 15)were proved by Southern hybridization, which prove them to be 16SrDNA of Helicobacter. (2)In situ hybridization results demonstrated highly positive rates in HCC and gastric cancer groups, of which were 60% and 73.3% respectively, and there was no statistics meaning between them; Non-HCC and leiomyome of uterus both were negative, only one of carcinoma of colon and rectal was positive, HCC group had statistics meaning with the three groups respectively. Furthermore , we detect 16SrRNA of Hh, but it had no positive result. (3)Finally all the positive of HCC were sequenced and theresult proved that they had high idensity(97. 8%) with H. Pylori and low idensity (80. 9%)with H. Fennellia.Conclusion: (1)First PCR and Southern hybridization show that 16SrRNAgene is detected in HCC and gastric cancer. (2)Secondly , in situ hybridization and sequence analysis prove that all the positive are related to Hp and it suggests that there is the relationship between the infection of Hp and HCC. (3) Neither of the 16SrRNA gene and 16SrRNA-mRNA is detected in HCC group.