Animal Model Of Oxygen-induced Neovascularization Retinopathy And Expression Of VEGF

Objective Neovascularization retinopathy such asdiabetic retinopathy, retinal vein occlusion and retinopathy of prematurity etc. may lead to visional decrease or loss. Because of pathogeny complex and indefintude mechanism , it is vital to establish suitable animal model so to evaluate the pathogenies and therapeutic approachs of neovascularization retinopathy. We established a set of device to induce retinal neovascularization in mice by ourselves according to relative documents and observed happening and regression of neovascularization and expression of vascular endothelial growth factor(VEGF), meantimes estimated methods and results of study .Method Twenty-four one-week old mice both female and male were equally grouped two at random. Twelve one-week old mice were exposed to 75% oxygen for 5 days and then to room air and oxygen densities in vessel were detected. Age -matched twelve mice without high oxygen exposure were used as controls. Opening oxygen vessel and replacing underlayed materials , feeding and displacing mother mice every one day. Sunlight illumination was taken and environmental temperatureretained 23 C 2 C. After animal models were established , mice were killed, and eyeball samples were parafinnembedded.HE staining , VIII factor antibody and VEGF immunocytchemistry were performed on some of sagittal cross-sections. Results Constriction and occlusion of the retinal blood vessels and decrease in perfusion were found under the hyperoxia condition, and dilation and proliferation of blood vessels were found under the relatively hypoxia status.1.The mean neovascular nuclei per cross-section was less 1. 038 0.679 in (he normoxia control,while 49.00 5.682 in hyperoxia group(t test, P<0.001) .2.Immunocytchemistry staining showed VIII factor antibody positive cells localized in thevitreal side of the inner limiting membrane in retinal sections of oxygen-treated eyes.3.Immunocytchemistry staining showed VEGF positive response from postnatal day 17 to day 21 in hyperoxia group,while negative responses in the normoxia control. Conclusion 1 .Thereproducible and inexpensive mice model of oxygen-induced retinal nevoascularization may prove to be useful for the study of pathogenesis of retina! neovascularization and medical intervention .2.HE staining showed markly retinal inner limiting membrane and vessel endothelial nuclei ,and counting was easy and convinent.3.Retinal spreading-slices may observe vessel growth and shape .4. vessel endothelially specialVIII factor antibody demonstrated cells which localized in the vitreal side of the inner limiting membrane were indeed endothelial cells of retinal nevoascularization. 5.1mmunocytchemistry staining showed VEGF remarkable positive responses from postnatal day 17 to day 21 and negative responses after 22d in hyperoxia group,while negative responses in the normoxia controls.