The RPE Transfected With Rac1-siRNA Vector In Vitro

PurposeRetinal neovasculariton and its associated complications are the most common reasons of blinds of many ocular diseases. Studies show it is a compensatory reaction following retinal ischemia and hypoxia. To investigate the effect of Rac1, trasfect Rac1-siRNA vector on the RPE in the hypoxia environmet. The understanding of the effect of Racl in vitro through a series of examination shed light on the treatment of a serial of diseases related retinal neovascularization.Methords1. Culture RPE, when the cells grow almost half, add the Lipofectamine 2000 mediated Rac1-siRNA vector. After 24h of culture, investigate the morphological character of the RPE and the transfected rate.2. Use the same methords transfect the vector, and then treat it with CoCl₂ for 24h. Investigate the morphological character and calculate the transfected rate through the fluorescent microscope.3. The proliferation rate of endothelial cells are assayed with MTT methord after 24,48,72 and 96h.