Researching Of A Kind Of Bioreactor Used To The Culture Of Tissue Engineering

Based on the development of the bioreactor used to the culture of tissue engineering in the nation and the overseas, this thesis analyzed the ulterior fluid field and the shearing stress distribution of the rotating wall bioreactor by using the finite element method, discussed the mass transfer process between the bioreactor and the culture and the infection of the impulse press. Based on the computer simulation and the theoretically analysis, we have designed and manufactured a rotating wall perfusion bioreactor system with interior circulation, and tested its capability with correlative experimentations.Through the theoretically analysis the vector figures of the shearing stress and the moving track of the culture within the rotating wall bioreactor have been given out. We also built a finite element model about the rotating wall bioreactor interior fluid field, calculated the shearing stress' values and the distribution status of the cultures, described the fluid velocity vector figure of the bioreactor space, and compared with the theoretically analysis results, the research results were primely consistent with them.We also analyzed the mass transfer process between the bioreactor and the culture, found some key factors which evidently affected the ability of the mass transfer. We proved that proper impulse press could enhance the mass transfer, and we could design a kind of high mass transfer bioreactor by the impulse press.Based on the theoretically analysis and the finite element simulation, we have designed a rotating wall bioreactor system with interior circle, offering gas and medium on-line and impulse press device. This bioreactor can offer a culture space about 700ml. The bioreactor can culture micro-carrier and cell/tissue scaffold.The testing results of the bioreactor system indicated that our bioreactor has a better ability to culture the scaffold with cells: hydroxy-phosphorite scaffolds and cancellous bone with cells which cultured our bioreactor system proliferated very markedly; cells cultured in it about 14 days have better modality than cultured in the 5%Researching of the bioreactor used to the culture of tissue engineering _ Abstractincubater.