The Protection Effects Of Superoxide Dismutase On The Irradiated Immunocytes From Organism Bearing Carcinoma

Objective: to research superoxide dismutase (SOD) action on lymphocyte lipid excessive oxidation of mice bearing Lewis pulmonary carcinoma after radiation , and the action on human lymphocyte number, proportion and lipid excessive oxidation. Try to clarify SOD radiaton resistance for lymphocyte and the protection on imrmmological function reduction after radiation.Material and method: 3 random groups of mice bearing Lewis pulmonary carcinoma include contrast group, radiation group, SOD + radiation group. The second one and the third one received total radiation, 6MeV X ray,5Gy by medical linear accelerator. The third one were injected SOD into abdominal cavity 30 minutes before radiation. After radiation, immediately draw the spleen. 752-type spectro-light degree meter detect OD value of the mice spleen lymphocyte, then we calculate MDA, T-SOD, GSH, GSH-PX, CAT in the spleen. Untreated small cell lung cancer patients diagnosticated by pathology were collected venous anticoagulation blood. The blood samples were separated 3 shares as the similar mice groups, received similar treatment and detection as mice samples. Result: Compared with the unradiation control group, the mice control group MDA in spleen rise obviously (p<0.05), but all the T-SOD, GSH,GSH-PX, CAT values reduce (p<0.01). The SOD + radiation group MDA value reduce, T-SOD, GSH, GSH-PX, CAT values rise (p<0.05), compared with radiation group. The radiation group MDA value rise T-SOD, GSH, GSH-PX, CAT values reduce (p<0.05), compared with control group. The SOD + radiation group MDA value reduce (p<0.05), T-SOD, GSH, GSH-PX, CAT values similar (p>0.05), compared with radiation control group. Experiment in vitro tell us that radiation make T-SOD, GSH in human serum reduced, MDA rose. And SOD makes an adverse result. SOD can relieve lipid peroxidation by radiation, reinforce body tolerance to radiation.Conclusion: 1. Radiation can reduce the mice spleen antioxidase, GSH-PX,GSH,CAT, rise free radical media products MDA. SOD product adverse result, can protect the harm from radiation. 2. Radiation can reduce T-SOD activity and GSH value in lung cancer blood, while rise MDA value. SOD product adverse result, can protect the harm from radiation.