| Objective: To observe the effects of all trans retinoic acid on human endometrial adenocarcinoma cells in vitro and to evaluate the possibility of its application in the treatment of human endometrial adenocarcinoma .Methods: The human endometrial adenocarcinoma cells (RL95-2) cultured in vitro were treated with various concentrations of all trans retinoic acid. At specific time points after treatment with all trans retinoic acid, the effect of all trans retinoic acid on the proliferation of RL95-2 cells was determined by MTT method, and Cyclin A, a material being concerned with cell cycle, and MMP-9, an enzyme marking the invasion were detected by immunohistochemistry,respectively. The morphological change of RL95-2 cells was observed by scanning electron microscope and transmission electron microscope. Results:(1) MTT analysis showed that the proliferative activity of RL95-2 cells after treatment with ATRA for 48h is negatively related to the concentration and treatment time of ATRA (r=-0.997ï¼›p<0.05), but this phenomena wasn't observed after ATRA treatment for 4 days.(2) FCM showed that ATRA exerts the effects on arresting the proceeding of RL95-2 cell cycle with a dose-dependent decrease in the percentage of S- and G2/M-phase cells and a concomitant increase in the percentage of cells in the G0/G1-phase of cell cycle, but this effect can only be detected after treatment with ATRA for 48h.(3) Markedly decrease in CyclinA and MMP-9 immunoreactivity was demonstrated by immunohistochemistry after 48 h of ATRA treatment.(4) Morphological changes suggesting cells differentiation were observed with TEM and SEM after 48h of ATRA treatment.Conclusion: ATRA has briefly inhibition effect on the proliferation, the natural cell cycle and the invasion capability of RL95-2 cells cultured in vitro, and further investigation is needed to determine whether ATRA is an effective drug for clinical use in the treatment of endometrial adenocarcinoma. ... |
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