The Development Of Preserved Boving Bone And Immunological Study Of Bone Implantation

Objective Large skeletal repair is always a tough problem perplexing surgeons. The bone graft substitutes play an important role in repairing bone defects, so the search for ideal bone graft substitute to repair bone defects is always an important subject in orthopaedics. Xenogeneic bone graftion is an altermative to autogeneic bone grafting, but the intense immune rejection makes its clinical practicelimited. the author implanted Preserved bovine cancellous bone, Preserved bovine cortical bone, Fresh bovine cancellous and Antigen-extracted bovine cancellous bone carrier in rat muscle pouch to investigate how about the immune rejection of Preserved bovine bone.at the same time.the author studied the compressive strength of all kinds of bone abovely.methods We performed lymphocytes proliferation assay, enzume-linked immunosorbent assay, and histological observation to evaluate the levels of cellular and humoral immunity, and the tissue reaction to the grafting of Wister rat receiving frech boving cancellous bone(FCB), Antigen-extracted bovine cancellous bone carrier(BCB), Preserved bovine cancellous bone(PBCCB) and Preserved bovine cortical bone(PBCTB), while nothing implantion was kept untreated as a control. After implantation ,we harvested at 1, 4, 12weeks. The biomechanics properties of all kinds of bone were evaluated by biomechanics testing instrument. Results Biomechanics test result showed that there was significant differemce inboth BCB and FCB and PBCB. Lymphocyte proliferation was increased in an early phase of the grafting and persisted for a long period in FCB group. Meanwhine higher levels of specific antibody production were not ascended and no appreciable histologic signs of immune or foreign body reaction both in BCB and PBCB groups. The results suggested that BCB and PBCCB were characterized less antigenicity and can be used as osteoconductive material of bone growth factors. Conclusion All the results suggested that PBCCB might be an ideal replacement material for bone transplantation, and might be used effectively as a kind of carrier of BMP. however ,its biomechanics intensity and how to combined with an approperiate dose of the BMPs needed further improving.