| Objective: Reactive oxygen species(ROS) has been implicated in the pathogenesis of ischemic stroke and atherosclerosis. NADPH oxidase, a major source of ROS generation in neutrophils and the vascular system, plays a critical role in ischemic injury and atherogenesis. Recently, an association between the C242T polymorphism of p22phox , an essential component of NAD(P)H oxidase, and coronary artery disease (CAD) and ischemic cerebrovascular disease (ICVD) has been reported in several studies. To investigate the relationship between the C242T polymorphism of p22phox and ICVD in Chinese patients , we conducted a case-control study.Methods: 112 ICVD patients (36 cases of TIA and 76 cases of cerebral infarction) and 105 control subjects were recruited and the C242T polymorphism of p22phox gene was analyzed by polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP). The measurement data was analyzed statisticly by t-test and enumeration data by chi-squere test.Results: The average age of ICVD group and control group (64. 92 11. 16 and 63.91 11.96 ,respectively) is of no difference (P = 0.523). The percentage of male between two groups has no significant difference (xl = 0.52, P = 0. 472) . The history of hypertension, diabetes, hyperlipodemia and smoking in ICVD group are all higher than control group(P<0.05). The TC genotype frequencies in the ICVD group and control group were 0. 152 and 0. 057, respectively, and no TT genotype was found. The prevalence of the TC genotype was significantly higher in the ICVD patients (P =0. 024, OR 2. 95, 95% CI 1. 12-7,81). Analysis by ICVD subtypes showed that the TC genotype frequency was of no difference between TIA and controls but was significantly differentbetween cerebral infarction and controls.Conclusions: The C242T polymorphism of the NADPH oxidase p22phox gene is implicated in the pathogenesis of ICVD and may be a risk factor for ICVD independent of hypertension, diabetes, hyperlipodemia and smoking .
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