Association Of C242T Gene Polymorphism With NAD (P) H Oxidase P22phox Subunit And Stroke

Background Stroke was one of the most common neurological diseases with a high mortality and morbidity rate. Recently, a lot of studies showed that oxidative stress caused by reactive oxygen species(ROS) had a close contact with the occurrence and development of stroke. As a major source of ROS generation in endothelia cell and vascular smooth muscle cell, NADPH oxidase played a critical role in electron transportation and producing the superoxide anion. NAD(P)H oxidase p22phox C242T coding gene polymorphism led to increase ROS generation, on one hand, redundant ROS maybe accelerated atherogenesis and the development of hypertension by direct oxygenation and indirect acting as second messenger to participate in signal transmission, which led to increase the susceptibility of stroke; on the other hand, redundant ROS maybe aggravated the lesion of brain tissue by direct injury and indirect acting as second messenger to mediate apoptosis in stroke. So the correlation between the polymorphism of the NAD(P)H oxidase p22phox subunit and stroke was causing more and more attention.Objective To investigate the relationship between the NAD(P)H oxidase p22phox C242T gene polymorphism and stroke.Methods 118 cases of cerebral hemorrhage,125 cases of cerebral infarction and 147 healthy controls were included in the study. Objects examined came from Han people in Shanghai region of China. Three groups had same baseline features of age, sexual proportion and BMI. Fasting glucose, total cholesterol, triglyceride were tested. DNA was isolated from every blood sample. The genotype and allele frequencies of the p22phox C242T polymorphism were determined by polymemse chain reaction, restriction fragment length polymorphism(PCR-RFLP) and gene sequencing technology. The statistic methord was t-test for measurement data and chi-squere test for enumeration data. Binary logistic regression analysis was used to select the risk factors of stroke.Result The CT+TT genotype frequencies in the cecebral hemorrhage group, cerebral infarction group and control group were9.3%,9.6% and 2.0%, T allele frequencies were 4.7%,5.2%and 1.0%, respectively. The CT genotype and T allele frequency of the cerebral hemorrhage group and cerebral infarction group ware significant higher than that of the control group (P<0.05), one TT genotype was found in cerebral infarction group, and no TT genotype was found in cecebral hemorrhage group and control group, which was consistent with the results of gene sequencing. Binary logistic regression analysis was used to determine the correlation of CT genotype, cerebral hemorrhage(P=2.114, OR 8.282,95% CI 1.484～46.200, P=0.016) and cerebral infarction (β=1.432, OR 4.187,95% CI 0.934-18.774, P=0.061) respectively.Conclusion These results suggest that the NAD(P)H oxidase p22phox C242T polymorphism was associated with cerebral hemorrhage and cerebral infarction, maybe serve as a risk factor. But compared to the traditional dangerous factors such as hypertension, diabetes, alcohol and carotid arteriosclerosis, the affect of the C242T polymorphism to the cerebral infarction was not significantly.