| Objective In order to find a simple,rapid and efficiency ways to get dendritic cells. K562 cell lines were induced to be dendritic cells by only calcium ionophore(A23187) ,A23187 combined with GM-CSF ,or cytokines cocktails including GM-CSF, IL-4 and TNF- a .Methods K562 cells were plated at 5 105/ml in 24-well cluster plates,andwere seperatedly cultured in medium for 7days with combinations of cytokines as follows:1) A23187 250ng/ml 2)A23187 180ng/ml and rhGM-CSF500U/ml 3) rhGM-CSF1000U / ml,rhIL-4 500U/ml and rhTNF- a 200U/ml for test groups. All cltures were maintained at 37 C in 5% CO2 and a humidified atmosphere.Every other day half dose of the inducing agents were added to test wells. Cultured cells were observed by phase-contrast microscopy for the change of morphology. The suface molecule CD1a,HLA-DR, CD54, CD83, CD80 and CD86 of induced DCs were detected by flow cytometry.The difference of expression rates before and after induction can be identified by this means. MTT colorimetry was used to test the ability that K562-DCs stimulated proliferation of allogenetic naive T cells and their induced cytotoxicity of T cells.Results DCs can be generated from K562 cells by single A23187 or combined cytokines. We found the group of A23187 acquired more DCs than the group of cytokines without A23187 within 72 hours (P<0. 05) .Respectively, the percentage of DC-like cells : (68.96 11.38) %, (76.78 11.12) %, (22.05 3.98) %. In addition ,both groups of DCs were high expression of CD1a, CD54,CD80,HLA-DR,CD83 and CD86., and had no evidence difference in terms of stimulatory abilities towards allogenetic T cells and their induced cytotoxicity of CTL(p>0.05).Conclusions Compared with those which are induced by combined cytokines,A23187 can induce K562 cell differentiating to DCs more rapidly and effectively than combined cytokines without A23187. DCs derived from K562 cell induced by A23187 present almost the same morphology, phenotype and functions.
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