The Study Of The Mutation Of Mismatch Repair Gene HMSH2 And HMLH1 In Hepatitis B Patients

Objective: To detect the mutation frequency of exon5 and exon12 of HMSH2 and hMLHl gene,investigate the relationship between the mutation of hMSH2N hMLH1 gene and the progress of hepatitis B, explore the pathogenesis of human hepatitis B.Methods: Genome DNA of 40 patients with hepatitis B, 17 patients with cirrhosis and 20 normal samples was subtracted, exon5 and exon12 of hMSH2 and hMLHl was amplified with PCR. The result was analyzed with SSCP.Results:(1)Mutations were identified in 1/40 hepatitis patients while there are no patients in normal people. There were no significant difference in the mutation rate of hMSH2 and hMLHl gene(P>0.05); (2)Mutations were identified in 3/14 cirrhosis patients while there are no patients in normal people. The mutation rate of hMSH2 and hMLHl gene in the cirrhosis patients was significantly higher than that in normal people. (P<0.05); (3) The mutation rate of hMSH2 and hMLHl gene in the cirrhosis patients was significantly higher than that in hepatitis patients(P<0.05).Conclusions: (1) There were no significant difference in the mutation rate of hMSH2 and hMLHl gene in hepatitis patients and normal people; (2) The mutation rate of hMSH2 and hMLHl gene in the cirrhosis patients was significantly higher than that in normal people,which indicate that the mutation of hMSH2 and hMLHl gene is closely associated with the pathogenesis of the cirrhosis;(3) The mutation rate of hMSH2 and hMLHl gene in the cirrhosis patients was significantly higher than that in hepatitis patients,which indicate that the mutation of hMSH2 and hMLHl gene does some use in the development from hepatitis B to cirrhosis.Postgreduate Wang li(Genetics) Directed by Wang peilin Wang xiuhai...