The Establisment Of Traumatic Optic Neuropathy Animal Model

[Objective] Traumatic Optic Neuropathy(TON) was increasing with the traffic accident. The methods of diagnosis and therapy were variable,the consents and the results were not as same as each other and all these were not formed the unity,lacking the basis foundation especially in the study of animal model.We began with establishing TON model and apply the objective examination methods to provide references to the clinical for its mechanism , checking standard , diagnosis and therapy and prognosis. [Methods] 2 rabbits and 2 cats who were health and growned up were killed.Whose nasal cavity, sinus and optic nerve were observed.Domestic cat was the ideal experiment animal.8 cats who were health and growned up were selected.The right eyes were selected as the experiment eyes and the left eyes were selected as the control's.Pattern Visual Evoked Potential(PR-VEP)was examined before damage.Through the nasal cavity sieve and sphenoid sinus,optic nerve of the pipeline was damaged by the injury tool which was made by ourselves.And animal models of TON were established,the PR-VEP was examined again by which we determined the succeed model.PR-VEP were examined at1h. 1d, 3d, 1w, 2w, 4w after damage.[Results] The paranasal sinus construction and optic nerve of the cat resembled with mankind,the nerve tube was completely and cat was the ideal experiment animal.8 cats's N75P100, P100N145 amplitude reduced and N75 , P100, N145 peak time prolong at 1h, 1d, 3d, 1w after damage. The N75 P100'S, P100N145's average amplitude respectively were 8.60 1.32uv, 7.90 0.39uv, 6.82 1.22uv, 3.40 0.43uv and 6.00 2.45uv, 4.50 0.93uv, 6.60 0.54uv, 3.00 0.91uv.The N75's, P100'S, N145'S average peak time resp-ecttively were 82.80+0.69ms, 120.60+1.36ms, 174.00 2.94ms, 106.20+7.14 ms, 136.80 6.17ms , 178.20 7.28ms,101.40 5.68ms , 144.60 7.28ms , 191.40 11.34ms,106.80 11.93ms, 148.80 14.21ms, 202.80 14.40ms.2w's and 4w's PR-VEP waves respectively were smooth threads.PR-VEP before operation comparing to 1h, 1d, 3d, 1w after operation were P<0.05, P<0.05, P,0.05, P<0.05 . PR-VEP of 1h comparing to 1d, 3d, 1w were P<0.05,1d, 3d, 1w compared each other were P>.05 or P<0.05.[Conclusion]This animal model was similar to clinical optic nerve decompression through nasal cavity , sieve and sphenoid sinus.The change of PR-VEP was distinctively before damage and after damage.This showed that this model was successful.And the PR-VEP of different time was different after damage.This was correlated with the effect which was caused by optic nerve decompression operation after injury in different time.The pupil reflects to the light was not proper examination standerd for TON animal model establish the success or not.