The Experiment Studies On The Effect Of The Expression Of Collagen Type Ⅰ And Type Ⅱ MRNA In Fature Rabbits With GTX Capsule

Objective: To investigate effect of fraction of GTX capsule on collagen type I and type II gene expression in the process of fracture union, and to explore the mechanism of GTX inenhancing fracture healing .Methods: Standardized radial factures were performed in New Zeland White rabbits, which were randomized into three groups: the GTX capsule group, the GZCSS group (the positive control group) , and the blank control model group. They were fed orally with GTX, GZCSS, and normal saline respectively, and were sacrificed on 1st week, 2nd week and 4th week after fracture in batches. The femur head was taken or detecting expression of collagen type I . II mRNA with situ hybridization, immuno-histological staining and pathologic examination. All data were compared with that of the positive control group and the blank control model group.Result: On 1st week, 2nd week and 4th week after fracture , The collagen type I and its mRNA expression of the GTX capsule group enhance without exception. And is obviously superior to the positive control group and the blank control model group (p<0.05). Compared with the positive control group and the blank control model group, the collagen type II and mRNA expression of the GTX capsule group rises clearly on 1st week,but declines on 2nd week and 4th week (p<0.05).Conclusion: The GTX capsule and the GZCSS can both adjust the expression of collagen concerned in the process of fracture union,and promote fracture union; The GTX capsule is superior than the positive control group and the blank control model group in the expression of collagen type I and II.