Studies On Chemical Constituents Of Polygala Tenuifolia Wild. And Its Pharmacological Activities

In order to find out more chemical constituents and its pharmacological activities in Polygala tenuifolia Wild. In this paper, we had a comprehensive research on it. The methods and results as follow:The dried roots of Polygala tenuifolia Wild. were extracted three times with boiling water. The extracted solution was passed through macroporous resin column and the adsorbed material eluted successively with H2O, 30%EtOH aq. and 85% EtOH aq. The 85% alcohol solution were collected and concentrated with reduced press and the general extraction were obtained. Twelve pure compounds were isolated from the general extraction by using silica gel chromatography, prep.TLC, active carbon decoloration, crystallization et al.The structures of the compounds had been identified on the basis of chemical and spectroscopic evidence(1HNMR, 13CNMR, HMBC, HMQC et al.). They are tenuifoliside A(Ⅰ),α-D-(6-O-sinapoyl)-glucopyranosyl(1→2)-β-D-(3-O-sinapoyl)-fructofuranose(Ⅱ),α-D-(6-O-4-methyl-3,5-dimethoxycinnamoyl)-glucopyranosyl(1→2)-β-D-(3-O-sinapoyl)-fructofuranose(Ⅲ), 3,4,5-trimethoxycinnamylic acid(Ⅳ),phenyl acetate(Ⅴ),nonane(Ⅷ)ethyl benzoate(Ⅵ),n-dodecane(Ⅶ). In which compound Ⅲ is a new constituent, other compounds but for compoundsⅠ,Ⅱ,Ⅳ were isolated from Polygala tenuifolia Wild. for the first time.At the same time, the chemical constituents in volatile oil from Polygala tenuifolia Wild. was analyzed by GC-MS-Computer technology. The results showed that the volatile oil contained at least 55 compounds, in which 18 compounds were identified with the computer standard MS spectra. The major compounds were Hexanoic acid, Phenylethy Alcohol, n-Hexadecanoic acid,Octadecanoic acid, 2-Methoxy-4-vinylphenol, Hexadecanoic acid and 2-hydroxy-(1-hydroxy methyl)-ethyl ester. In which their relatively quantities are 21.52%, 6.19%, 4.00%, 3.09%, 1.94% and 1.40% respectively.The pharmacological activities of the general extraction from Polygala tenuifolia Wild. were studied. The results showed that the general extraction have the function of removing phlegm and accelerating gastrointestinal peristalsis.