| Ischemic heart disease has become main disease hurt human healthy in whole world. To search safe and effective drugs preventing and curing ischemic heart disease is an important task of medicine research.Injection of Ginseng fruit saponins (IGFS) was separated from fruit of Panax ginseng(C.A. Mey.). Main component of IGFS is Ginsenside-Re and its content has reached 85 percent. The protevtive effect of IGFS on experimental myocardial infarction in rats and its mechanism analysis were reported in this paper. The rats were randomizied to five groups: group of sham operation(ip normal saline 2m1/kg.d×3d after sham ligation), group of model (ip normal saline 2m1/kg.d×3d after ligation),and three dose groups of IGFS (ip IGFS 10mg/kg,20mg/kg and 40mg/kg.d×3d after ligation). Acute myocardial infarct model(AMI) was established as follows:After anesthetised with ether, rats were face upward fixed on the operating table . The chest was opened between the left third and fourth rib and the heart was exposed. A nylon suture attached to a fine needle was placed under the left anterior descending coronary artery and ligated coronary artery immediately. After the heart was return into the chest, the blood and water in the chest were squeezed out and swiftly close the chest . the time opening the chest is no more than 30 seconds. sham surgical group placed the suture only but not ligate coronary artery. After 24 hours of ligating coronary artery, rats were anesthetized by 30% barbital sodium(30mg/kg,ip). The hearts were taken out and perfused with NS through aorta. Cut off atrium tissue and fat,crosscut myocardium of left ventricle into four or five slices, immerse them into the N-BT phosphate buffer in water bath at 37℃. Take them out after dying completely. Normal tissues were dyed while ischemic tissues not. MIS was expressed as the ratio of ischemic myocardium weight to left ventricle wet weight.Activities of serum creatine phosphokinase(CK),lactateDehydrogenase(LDH), aspartate aminotransferase(AST), superoxide dismutase (SOD) and glutathione peroxidase(GSH-Px),contentof lipid peroxidation(LPO), and levels of plasma endothelin(ET), angiotensinⅡ(AngⅡ), prostacycline(PGI2),thromboxane A2(TXA2) , and myocardial free fatty acid (FFA) content of infarct and noninfarct area were determined.Blood was collect to determine low shearing specific viscosity,middle shearing specific viscosity, high shearing specific viscosity, plasma specific viscosity. The results showed that In rats treated by IGFS (in a dosage of 10, 20 and 40mg/kg.d,i.p×3d ),the MIS was significantly reduced, the activities of serum CK ,AST and LDH , the levels of plasma ET, AngⅡ and TXA2 and the content of myocardial FFA declined, while the level of plasma PGI2 and ratio of PGI2/TXA2 were increased signficatly. In addition, the content of serum LPO and viscosity of blood and plasma were declined, activities of SOD and GSH-Px were increased markedly. What we observed suggest that IGFS have protective effect on myocardial ischemia by improving improving free radicals and myocardial metabolism etc. GFS, in our view, may be an effective drug for prevention and cure of myocardial ischemia.
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