| Objective :To study the effects of bone morphogenic protein-6( BMP-6 )on repairing defects of articular cartilage o BMP-6 were added into stabilized fibrin-chondrocyte constructs. The stabilized fibrin-chondrOcyte constructs were fibrin glue which were added in aprotinin and tranexamic acid.The stabilized fibrin -chondrocyteconstructs with BMP-6 were cultivated in vitro and proliferation of the cells on the bracket and degradation of bracket and secretion of extracellular matrix were observed. Methods 1. The chondrocytes from articular cartilage of 3 weeks old New Zealand rabbit were isolated and monolayer cultured . then the proliferation seed cells were seeded on the improved FG scaffold with BMP - 6 or not . The stabilized fibrin-chondrocyte constructs were culltured and amplined for 2 weeks in four-dimensional spatial in vitro. Histological and electron microscope were used to study the behavior of chondrocytes cultured with fibrin glue. The degradated speeds of fibrin-chondrocyte constructs were evaluated in difierent periods. 2. Two defects of articular cartilage were made in the trochlear groove of each new Zealand rabbit. The defect of group A was filled with stabilized fibrin-chondrocyte constructs with BMP-6 and t The defect of group B group was filled with stabilized fibrin-chondrocyte constructs without BMP-6. The defect of group C was filled only with stabilized FG scaffold. The group D was left empty .The repaired defects were periodically examined grossly and histologically. Result: 1. The cell multiplication time of the purecytoskeleton group spends 4. 7 days. The The cell multiplication time of 4.5 days, there was no significant difference in the cells growth speed between two groups .2.1n vitro,two weeks postcultured,there was significantly stastical difference of Glycosaminoglycan between two groups.3. Two weeks postoperatively, implanted cartilage blocks could be seen in al 1 experimental groups and all blocks stayed in the defect of articular cartilage,but The borders were clearly identified and none of implanted blocks had connected with circumference. A little extravasated blood could be found in the group D. 4. Six weeks postoperatively, untypical hyaline cartilage could be found in the bone morphogenic protein-6 group, and there were many cartilage-like cells , which arrayed regularly. The newly born cartilage has plenty toluidine blue staining male matrix, and few lymphocyte cells could be seen in some areas. The depth of all blocks reach as 2 / 3-3 / 4 high as normal articular cartilage. Their surface were smooth. Such phenomena could also be seen in the group B, but the depth of all blocks only reach the high of 1 / 2-2 / 3 to normal articular cartilage. And The newly born cartilage of the group B had less toluidine blue staining male matrix than that of the group A; and the cartilage cells of the group B arrayed less regularly. And its surface looked smooth. In the group C, fibrous tissue covered defected areas, the surface Of which were uneven and the borders were identified clearly, In the group D, the defected areas were covered by a thin sheet tissue mainly composed of fibrous scar and imflammation tissue. 5. Twelve weeks postoperatively, the implanted blocks hi thegroup A had tightly integration with vicinity cartilage, the defects of which were almost as high as normal cartilage. The newly born cartilage had nearly the same texture as the normal cartilage. The superficial layer cartilage cells had oval shape and the cartilage cells' major axis parallel with articular surface. 6. According to the Wakitani' s standard, the group A has the best plerosis effect on the repairing of articular cartilage defects compared with the other groups. It had been proved to have the significantly difference among all the control groups by statistics disposal. Conclusion : 1. In vitro, there was no evidence difference between the growth speed of two groups. 2. BMP-6 could significantly accelerate the secretion of extracellular matrix 3. BMP-6 applied to stabilized fibrin glue constr...
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