| Wound and various kinds of disease caused articular cartilage defect are influencing patient's quality of life. Therefore, orthopaedics doctors devote themselves to explore and consummate satisfied joint of dissect and function characteristic method for repair and rebuild for many years. Autologous, allogeneic cartilage and artificial materials had been used in clinical, but there's demerit such as material source, supply border defect and immunization exclude. It's possible to avoid defect of tradition cure method, and solve the difficulty of articular cartilage of repair and rebuild as the development of tissue engineering technology. There need great quantity of seed cells and fine cytoskeleton materials for tissue engineering to satisfy the request of engineering tissue of organ for building and moulding. Culture of seed cells is a basic element of tissue engineering, cells mainly come from self-cell, variant of the same homogeneity, cells of heterogeneity, and so on. Self-histiocyte is the first choice. Owing to cartilage tissue engineering often demands high concentration of inoculation cells, self-chondrocyte has some questions about quantity of localization, passed culture for long period of time and function aging.The engineering of stem cells was to get across to separation, purification, direct inducement, differentiation, a great deal of expand increase and conformity construct tissue or organ in vitro using modern biomedicine and tissue engineering technique.
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