| Background:Coronary heart disease (CHD) is the most cardiovascular disease which damaged peoples health in the days. In recent years along with technical development in medical treatment, the processed method be developped from pure medicine to dissolve to bol, percutaneous transluminal coronary angioplasty, stents transplanted, coronary artery bypass grafting (CABG), trans myocardial Laser revascularization(TMLR, myocardial beats bore etc. Experiment and clinical research of myocardial ischemia also be developed. Circulating vasoactive substances play a very importent role in the CHD and myocardial ischemia. Contractile vessel substances (Endothelin , Angiotensn II, noradrenalin )and relaxing vessel substances (Calcition gene related peptide, adrenomedullin, Atrial natriuretic peptide)play a very important function in the the pathologic physiology process of myocardial ischemia. These live materials in myocardial ischemia do good to rectifing myocardial ischemia and preventing the development of heart disease. Therefore there is necessity paying attention to the role of every kind of vessel substances and their related relation, and need to find out and study the new vessel substances in the myocardial. Urotensin-II (U-II) is a vasoactive somatostatin-like' cyclic peptide which was originally isolated from fish spinal cords, and which has recently been cloned from man. Here we describe the identification of an orphan human G-protein-coupled receptor homologous to rat GPR14 and expressed predominantly in cardiovascular tissue, which functions as a U-II receptor, human U-II bind to recombinant human GPR14 with high affinity,.Human U-II is found within both vascular and cardiac tissue (including coronary atheroma) and effectively constricts isolated arteries from non-human primates. The potency of vasoconstriction of U-II is an order of 4-16 fold magnitude greater than that of endothelin-1, making human U-II the most potent mammalian vasoconstrictor identified so far. Pepole Discovered that there is a high expression of UII in the part of coronary artery atherosclerosis. The study means UII may have effection on the development of coronary artery atherosclerosis.The biologic characteristic and distribution of UII and its receptor(UT-II)prvide basis of cardiovascular modulation, which showed that Ullmay be a role in prognosis of cardiovascular diseases. With the more study of biologic characteristic of UII, the clinical research also be gradually thorough. Domestic and international scholar in near period have studied relation between CHD and UII, but it is not enough meticulous completely. Furthermore, the combination research of clinical and basic is a few . Therefore, the first part of the study invetigated the variety of blood plasma UII of patients with CHD and the correlation between UII and other vessel substances .It proves that these vasoactive substances in patients with CHD .Although their changes are not the same, they participate in compensatory regulation of CHD, so it may provide theory basis and objective guideline for diagnosis and treatment of CHD. The second part studies the expression of UII in model of mouse with AMI. it provide the basis of the foundation research for the clinical result.Objective:1. We study changes of plasma levels of urotensin II in patients ith CHD and our aim is to illustrate clinical signification and action of urotendin II.2. By studying the relationship between urotensin II and the 20 peptides of proadrenomdullin N- terminal, we can further clarify that the changes of these vessel substances and their action in the process of myocardial ischemia and its interaction mechanism.3. We want to illuminate action during acute myocardial ischemia by qualitative analyzing the expression of UII in myocardial cell.Methods:1. Fifty patients with coronary heart disease(CHD) who coronary angiography indicated coronary stenosis and twenty healths were studied; Clinical group:the stable angina, unstable angina, AMI. coronary vessel cha...
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