| Objective: The purpose of this pilot study was as followed: (1)Bone marrow stromal cells isolated from dog treated with PRP which prepared from autologous blood and the effects of PRP on the induced osteoblast of the BMSCs were identified. (2) To explore the potential of using tricalciumphosphate and platelet-rich plasma for alveolar ridge bone healing. In search of an ideal bone grafting materials and substitutes. (3) To evaluate the effects of implantation of autogenous root and TCP/PRP in conjunction with guided bone regeneration(GBR) for alveolar ridge augmentation in the mongrel dogs. All what we did in this pilot study could provide enough theoretical evidence and experimental information for the new methods and technique of alveolar ridge augmentation.Methods: (1) Bone marrow stromal cells isolated from the mongrel dogs were maintained in Dulbecco's Modified Eagle Medium (DMEM) basic medium supplemented with 10% fetal calf serum (FCS) for the primary culture. Cellswere treated with PRP (10ml/L) prepared from autologous venous blood for the subculture.The morphological characters of BMSCs were observed with phase contrast microscope and ultrastructure were observed under electron microscope, the cellular DNA contents were observed with flow cytometer, then the alkaline phosphatase activities were determined to record the amount of osteocalcin in the culture medium.The mineralized nodules in the culture dishes were stained with saturated solution of Alizarin Red. (2) In each jaw quadrant of both sides, a preclinical model of alveolar defects was surgically produced, following the extraction of the sixth premolar teeth according to the surgical protocols. (3) 4 weeks later, alveolar ridge defects were induced and implant autogenous root and TCP/PRP in conjunction with polytetrafluoroe-thylene membrane (PTFE)for alveolar ridge augmentation. Two groups were divided, Experimental group: dental roots+TCP/PRP/ PTFE; Control group: dental roots+TCP/ PTFE. (4) The animals were euthanized after a healing period of 6 weeks and 12 weeks ,and the experimental sites were analyzed histologically and histomorphometrically.Results : (1) Bone marrow stromal cells were cultured well with addition of PRP (1%) in vitro. PRP altered the shape of cells and up-regulated cellular DNA contents at 72 hours. PRP's action on osteocalcin synthesis was detected, and the Alkaline phosphatase activities of the cells were observed, and the mineralized nodules in the culture dishes were stained by Alizarin Red histochemical staining methods. (2) Results from the animal studies have showned that the autogenous root in combination with B -tricalciumphosphate, platelet-rich plasma (PRP), and guided bone regeneration(GBR) could augment atrophic alveolar ridge. The outcomes were effective and theosseointegration between root implants and alveolar bone were achieved.. The experimental groups showed excellent healing results including the quantity and quality of newly regenerated bone bone in contrast to the control groups by histologic and radiographic evaluation. The height and width of newly regenerated alveolar ridge were: (3.17+0.53) mm ,(3.23+0.48 )mm and (2.61 +0.35)mm, (2.67+0.31) mm.Conclusions: (1) PRP could stimulate the synthesis of BMSCs DNA, and the expressions of ALP and the secretion amount of osteocalcin were increased greatly, PRP could promote BMSCs to differentiated into osteoblasts in vitro. (2) TCP/PRP could act as the bone graft materials in alveolar ridge augmentation procedures. (3) Autogenous root could be used for alveolar ridge augmentation, in combination with bone graft materials can be achieving osseointegration. (4) PRP had the ability to enhance bone regeneration and improve wound healing , which could be used to treat periodontal osseous defects as an adjuvant in clinical application. (5) A combination of autogenous root implants, platelet-rich plasma (PRP), tricalciumphosphate, and guided bone regeneration(GBR) had been shown to be effective in augmenting alveolar ridge bone. In our opinion, this t...
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