| IntroductionBirth control is an basic policy in our contry ,so the sutudy of the anti-pregnancy drugs is an important part in birth control programme.By now mainly clinical anti-pregnancy drugs are steroid-compound ones which having high position effect and always belonging to inhibiting-ovulation durgs.This kind of durgs have certain interference to organism.Traditional Chinese medicine have character of special nature effect ,low-position effect and little side effect.Extracting the effective portion of Verbena of offcinalis L ,preparating the anti-pregnancy portion have significant meaning to develop new drugs.Decidua is the necessary tissue to embedding of ovum and growth of embryo.Interfering the developing of deciduas should be the good anti-early pregnancy activity.Cell culture in vitro has the vitue of direction and Traditional Chinese medicine. Verbena of offcinalis L have urging menstruation and diuresis effect and be avoiding to pregnancy women . The successful abortion rate was 83.4% in 76 healthy early pregnancy(33~42day) women who had taken Verbena of offcinalis L power.Cluture of the trophoblast in vitro had shown thatVerbena of offcinalis L could inhibit the growth of the trophoblast and HCG secretion obviously.Animal experiment had shown Verbena of offcinalis L also could inhibit the developing of embryo and make it wither ,die;destroy the syncytiatrophoblast cells;could terminate the early pregnancy in pregnant mouse. However ,until now whether Verbena of offcinalis L can inhibit the growth of the decidual cell is obscure and there was no report of the effective portion of Verbena of offcinalis L in terminating early pregnancy internal.Traditional Chinese medicine have good anti-preganancy effect with little side effct and can be taken convinently.lt is being paid attention to in the world. Now-Adays, although some study of traditional Chinese medicine used in terminating pregnancy had been reported ,but few studies about its activity were on cell level.In this experiment,by means of culture of decidual stromal cells , the effect of diffirent extraction of Verbena of offcinalis L (A, B, C, D)and mefepristone on the morphology and viability, proliferation , apoptosis, cellcycle proliferation was be observed to explore machanism of Verbena of offcinalis L and mefepristone with anti-early pregnancy activity on cell level and investigate the active portion in the Verbena of offcinalis on anti-early pregnancy activity .PART 1Study on Culture of Human Decidul Stromal Cell in Vitro Objective To explore the simple and valuable method to culture human decidual stromal cell.Methods In this experiment ,abortion decidual tissues of earlypregnant healthy women were cultured in vitro. Compounding FD(F-12/DMEM, 1:1) compelete culture fluid , 0.25% trypsin and0.02%EDTA (1:1) .All components of decidual cells were culturedtogether. After culturing for 24 hours ,the culture fluid were changedand unadhesive decidual cells and red blood cells were got ridof.According to the growth of cells,changed the culture fluid every 2-3days. When merged to 80%,the cells were digested by 0.25%trypsin and 0.02%EDTA (1:1) . After transferring three times the DSCcould be isolated and purified. Made cell climbing flake.Then theDSC were identified by immunohischemistry (prolactin PRL).Result Human decidual stromal cells present typical elliptic shape andpresent shape like fiberblast when cultured in singlelayer. Afterinoculating ,30 minitue later the cells began to adhere to the wall andelongate to fiberblast shape gradually,presenting the longer shuttle shapeand irregular star shape. The cell nucleus presenting elliptic shape andwere in the center of the cells.The cell nucleous were distinct withabundant cytoplasm pellet.24 hours later cells mostly adhere to the walland 6~7 days later could merge .These cells could multiplicate for someweeks in 20%FCS culture fluid and kept unchanged state until merging.In these cell...
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