The Research Of Angiogenensis And Antiangiogenesis Factors In Lung Cancer

Objective: To probe into the clinical significance of correlative molecules expressions and the relation between molecules expressions and invasion > metastases, mirovessel density of lung cancer. At the same time, to analyze the impacts of different parameters and protein expressions on patients survival synthetically.Methods: To collect 83 lung cancer resection samples with complete clinical and prognostic data . To carry through CD34 imrnunohistochemistrical (IHC) staining and in slices and study the mirovesselgenesis characteristic and differences in different histological types of lung cancer according to microvessel density (MVD) and morphology. To carry out Cathepsin, Cyclooxygenase-2(COX-2, Vascular endothelial growth factor (VEGF), transformoing growth factor -1β(TGF-β1 proliferating cell nuclear antigen(PCNA) IHC staining and TUNEL staining and expound the mechanism of molecules functions and their correlative significance in patients prognosis. To establish COX regression model with patients' pathologiclim'cal data and molecules aforementioned and discuss their effects on patients prognosis.Results: The MVD count is significantly different between groups with or without lymph node metastases as well as in different clinical stages in these 83 lung cancer cases (P<0.05) and is negative-relative with patients' prognosis(P<0.01). The studies of IHC staining shows: MVD count is correlative with proto-angiogenensis substances' expressions secreted by tumor cell, while there are also significances between MVD count and molecules' expressions related with tumor invasion and metastases. We also found VEGF,Cathepsin-D, COX-2 -, TGF- β1 expressions are markedly higher in group with lymph node metastases than in group withoutmetastases. In addition, these expressions are negative-relative with patients' prognosis. The COX regression analysis of patients' survival according to factors above-mentioned indicate that the main influenceable factors are therapy means histological types TNM clinical classification, VEGPN TGF-3,, Cathepsin-D expressions, among which histological types and Cathepsin-D expression are far more important.Conclusions: lung cancer cells can excret cytokines to promote angiogenensis in order to satisfy requirements of fast growing and gain high invadable capacity and result in poor prognosis. The multivariate analysis of COX Regression model shows: clinical stage affects significantly patients' prognosis, the high clinical stage is, the lower survival rate is; moreover, the prognosis is poorer in patients suffered adenocarcinoma and small cell lung cancer than in patients with squamous cell carcinoma.