| BackgroundGestational diabetes mellitus is defined as carbohydrate intolerance of varying severity with onset or first recognition during pregnancy. Pregnancies complicated by this disease are associated with the risk of both maternal and perinatal morbidity. In order to improve the result of pregnancy, the early diagnose of this disease becomes very important. More and more investigators focus on the function of gene. Some of them considered that gestational diabetes mellitus has the same genetic characteristic as type 1 or type 2 diabetes. Type 1 and type 2 diabetes are associated with HLA-n gene, which has been reported by several studies. Whether GDM correlated to HLA-n alleles polymorphism and which gene was susceptible or protective are rarely reported. ObjectiveTo investigate the association between polymorphism of HLA-DQA1 and susceptibility of gestational diabetes mellitus. MethodsThis study enrolled 35 cases of GDM and 35 cases of normal pregnant women excluded diseases correlated with HLA gene. 2ml elbow venous blood was get and anticoagulated by EDTA. Genomic DNA was extracted from peripheral venous blood by TKM method. PCR-RFLP gene typing technique was used to detect HLA-DQA1 alleles. ResultsIt was observed that gene frequency of HLA-DQA1*0201 increased in GDM group compared with control group (22.86%vsl 1.43%, P<0.05). We also observed an increase of HLA-DQA1 *0102 , 0401 and 0501 gene in GDM group,but the statistical difference werenotsignificant(14.29%vs 11.43%,2.86% vs 1.43%, 18.57% vs 17.14%,P>0.05). Aslightly decrease in HLA-DQA1*0103, 0301 and 0601 was seen in GDM group (7.14% vs 10.00%,22.86% vs 25.71%,4.29% vs 7.14%,PX).05), an obvious decrease in HLA-DQA1*0101 (7.14% vs 15.71%,P=0.088), but the statistical difference were not significant. ConclusionsAlleles HLA-DQAl *0201 was significantly implicated in its susceptibility to GDM at Han ethnic of China, but which genotype acts as the protective gene is still unknown.
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