| Objective To investigate the immunosuppressive effect and related mechanism of Qinghaosu. Methods A modle of DTH induced by dinitrofluorobenzene(DNFB) in mice was established and the effect of various concentrations of Qinghaosu, FK506 ,FK506 combined with Qinghaosu on DTH response were observed. Murine auricle weight difference of left and right 24hours after stimulation was used to define DTH ,the local infiltration of mononuclear cell in the right ear was detected by HE(hematoxylin-eosin stain), the serum level of Interferon-gamma(IFN- y ) was measured by ELISA and the expression of intercellular adhesion molecule 1(ICAM-1) was tested by Immunohistochemistry . Results Excepting Qinghaosu 50mg/kg/d group, The murine auricle weight difference of left and right 24hours after stimulation, the infiltration of mononuclear cell, the level of Interferon-gamma(IFN- y ) in serum and the expression of intercellular adhesion molecule 1(ICAM-1) were significantly decreased in the other groups of different concentrations of Qinghaosu, FK506 and Qinghaosu combined with FK506, as compared with control group, and the effect of Qinghaosu was related to the dosage within a certain dosage range. Conclusion Qinghaosu has immunosuppressive effect on DTH in mice when the dosage is above 150mg/kg/d and is dose dependent within a certain dosage range, it can also be find that Q'inghaosu has a synegistic action with FK506. one of the mechanisms might be by inhibiting the production of IFN-γ in serum and downregulating the expression of local ICAM-1 in the ear.
|
PDF Full Text Request